PHYLOGENETIC ANALYSIS OF THEILERIA ANNULATA INFECTED CELL LINE S15 IRAN VACCINE STRAIN

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HABIBI GH.

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Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF PARASITOLOGY Year:2012 | Volume:7 | Issue:2 Page(s): 73-81

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Title

PHYLOGENETIC ANALYSIS OF THEILERIA ANNULATA INFECTED CELL LINE S15 IRAN VACCINE STRAIN

Author(s)

HABIBI GH. | Issue Writer Certificate

Keywords

THEILERIA

VACCINE STRAIN

PHYLOGENETIC ANALYSIS

PCR

18S RRNA GENE

Abstract

Background: Bovine theileriosis results from infection with obligate intracellular protozoa of the genus THEILERIA. The phylogenetic relationships between two isolates of THEILERIA annulata, and 36 THEILERIA spp., as well as 6 out group including Babesia spp. and coccidian protozoa were analyzed using the 18S RRNA GENE sequence.Methods: The target DNA segment was amplified by PCR. The PCR product was used for direct sequencing. The length of the 18S RRNA GENE of all THEILERIA spp. involved in this study was around 1, 400 bp.Results: A phylogenetic tree was inferred based on the 18S RRNA GENE sequence of the Iran and Iraq isolates, and other species of THEILERIA available in Gen Bank. In the constructed tree, THEILERIA annulata (Iran VACCINE STRAIN) was closely related to other T. annulata from Europe, Asia, as well as T.lestoquardi, T. parva and T. taurotragi all in one clade.Conclusion: Phylogenetic analyses based on small subunit ribosomal RNA gene suggested that the percent identity of the sequence of Iran VACCINE STRAIN was completely the same as Iraq sequence (100% identical), but the similarity of Iran VACCINE STRAIN with other T. annulata reported from China, Spain and Italy determined the 97.9 to 99.9% identity.

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APA: Copy

HABIBI, GH.. (2012). PHYLOGENETIC ANALYSIS OF THEILERIA ANNULATA INFECTED CELL LINE S15 IRAN VACCINE STRAIN. IRANIAN JOURNAL OF PARASITOLOGY, 7(2), 73-81. SID. https://sid.ir/paper/304013/en

Vancouver: Copy

HABIBI GH.. PHYLOGENETIC ANALYSIS OF THEILERIA ANNULATA INFECTED CELL LINE S15 IRAN VACCINE STRAIN. IRANIAN JOURNAL OF PARASITOLOGY[Internet]. 2012;7(2):73-81. Available from: https://sid.ir/paper/304013/en

IEEE: Copy

GH. HABIBI, “PHYLOGENETIC ANALYSIS OF THEILERIA ANNULATA INFECTED CELL LINE S15 IRAN VACCINE STRAIN,” IRANIAN JOURNAL OF PARASITOLOGY, vol. 7, no. 2, pp. 73–81, 2012, [Online]. Available: https://sid.ir/paper/304013/en

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