SEQUENCE ANALYSIS OF THE SECOND INTERNAL TRANSCRIBED SPACER (ITS2) REGION OF RDNA FOR SPECIES IDENTIFICATION OF TRICHOSTRONGYLUS NEMATODES ISOLATED FROM DOMESTIC LIVESTOCK IN IRAN

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GHASEMIKHAH R.; SHARBATKHORI M.; MOBEDI I.; KIA E.B.; FASIHI HARANDI M.; MIRHENDI H.

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Journal: IRANIAN JOURNAL OF PARASITOLOGY Year:2012 | Volume:7 | Issue:2 Page(s): 40-46

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Title

SEQUENCE ANALYSIS OF THE SECOND INTERNAL TRANSCRIBED SPACER (ITS2) REGION OF RDNA FOR SPECIES IDENTIFICATION OF TRICHOSTRONGYLUS NEMATODES ISOLATED FROM DOMESTIC LIVESTOCK IN IRAN

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Keywords

TRICHOSTRONGYLUS

ITS2

IRAN

LIVESTOCK

Abstract

Background: Infectivity of herbivores with TRICHOSTRONGYLUS nematodes is widespread in many countries, having a major economic impact on breeding, survivability, and productivity of domestic LIVESTOCK. This study was carried out on TRICHOSTRONGYLUS species isolated from domestic LIVESTOCK in order to develop an easy-to-perform method for species identification.Methods: TRICHOSTRONGYLUS isolates were collected from sheep, goat, cattle, and buffaloes in Khuzestan Province, southwest IRAN. Primary species identification was carried out based on morphological characterization of male worms. PCR amplification of ITS2-rDNA region was performed on genomic DNA and the products were sequenced. Phylogenetic analysis of the nucleotide sequence data was conducted employing Bayesian Inference approach. Consequently, a restriction fragment length polymorphism (RFLP) profile was designed to differentiate TRICHOSTRONGYLUS species.Results: A consensus sequence of 238 nucleotides was deposited in the GenBank for IRANian isolates of TRICHOSTRONGYLUS species including T. colubriformis, T. capricola, T. probolurus and T. vitrinus. The designated RFLP using restriction enzyme Tas I could readily differentiate among species having different ITS2 sequence. The molecular analysis was in concordance with morphological findings.Conclusion: Phylogenetic analysis indicated a close relationship among the sequences obtained in this study and reference sequence of relevant species. ITS2-RFLP with Tas I is recommended for molecular differentiation of common TRICHOSTRONGYLUS species.

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APA: Copy

GHASEMIKHAH, R., SHARBATKHORI, M., MOBEDI, I., KIA, E.B., FASIHI HARANDI, M., & MIRHENDI, H.. (2012). SEQUENCE ANALYSIS OF THE SECOND INTERNAL TRANSCRIBED SPACER (ITS2) REGION OF RDNA FOR SPECIES IDENTIFICATION OF TRICHOSTRONGYLUS NEMATODES ISOLATED FROM DOMESTIC LIVESTOCK IN IRAN. IRANIAN JOURNAL OF PARASITOLOGY, 7(2), 40-46. SID. https://sid.ir/paper/304019/en

Vancouver: Copy

GHASEMIKHAH R., SHARBATKHORI M., MOBEDI I., KIA E.B., FASIHI HARANDI M., MIRHENDI H.. SEQUENCE ANALYSIS OF THE SECOND INTERNAL TRANSCRIBED SPACER (ITS2) REGION OF RDNA FOR SPECIES IDENTIFICATION OF TRICHOSTRONGYLUS NEMATODES ISOLATED FROM DOMESTIC LIVESTOCK IN IRAN. IRANIAN JOURNAL OF PARASITOLOGY[Internet]. 2012;7(2):40-46. Available from: https://sid.ir/paper/304019/en

IEEE: Copy

R. GHASEMIKHAH, M. SHARBATKHORI, I. MOBEDI, E.B. KIA, M. FASIHI HARANDI, and H. MIRHENDI, “SEQUENCE ANALYSIS OF THE SECOND INTERNAL TRANSCRIBED SPACER (ITS2) REGION OF RDNA FOR SPECIES IDENTIFICATION OF TRICHOSTRONGYLUS NEMATODES ISOLATED FROM DOMESTIC LIVESTOCK IN IRAN,” IRANIAN JOURNAL OF PARASITOLOGY, vol. 7, no. 2, pp. 40–46, 2012, [Online]. Available: https://sid.ir/paper/304019/en

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