TRICLABENDAZOLE EFFECT ON PROTEASE ENZYME ACTIVITY IN THE EXCRETORY- SECRETORY PRODUCTS OF FASCIOLA HEPATICA IN VITRO

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SHRIFI YOSEF; FARAHNAK ALI; GOLESTANI ABOLFAZLE; ESHRAGHIAN MOHAMADREZA; FARIDI ASHKAN; MOLAEI RAD MOHAMAD BAGHER

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Journal Paper

Paper Information

Journal: Iranian Journal of Parasitology Year:2014 | Volume:9 | Issue:1 Page(s): 107-113

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Title

TRICLABENDAZOLE EFFECT ON PROTEASE ENZYME ACTIVITY IN THE EXCRETORY- SECRETORY PRODUCTS OF FASCIOLA HEPATICA IN VITRO

Author(s)

Keywords

F. HEPATICA

EXCRETORY-SECRETORY PRODUCT

PROTEASE

TRICLABENDAZOLE

Abstract

Background: Fasciola hepatica is one of the most important helminthes parasites and TRICLABENDAZOLE (TCBZ) is routinely used for treatment of infected people and animals. Secreted PROTEASE enzymes by the F. HEPATICA plays a critical role in the invasion, migration, nutrition and the survival of parasite and are key targets for novel drugs and vaccines. The aim of study was to determine the PROTEASE activity of excretory- secretory products (ESP) of F. HEPATICA in the presence of TCBZ anthelmintic.Methods: F. HEPATICA helminthes were collected and cultured within RPMI 1640 [TCBZ treated (test) and untreated (control)] for 6 h at 37 oC. ESP of treated and control were collected, centrifuged and supernatants were stored at -20 oC. Protein concentrations were measured according to Bradford method. PROTEASE enzymes activities of ESP samples were estimated by using sigma's non-specific PROTEASE activity assay. ESP protein bands were detected by sodium dodecyl sulfate poly acrylamide gel electrophoresis (SDS-PAGE).Results: Mean protein concentrations in control and treated of ESP samples were determined 196.1±14.52 and 376.4±28.20 mg/ml, respectively. Mean PROTEASE enzymes activities in control and treated were 0.37±0.1 and 0.089±0.03 U/ml, respectively. Significant difference between proteins concentrations and PROTEASE enzymes activities of two groups was observed (P<0.05). SDS-PAGE showed different patterns of protein bands between treated and control samples.Conclusion: The TCBZ reduced secreted PROTEASE enzymes activities and possibly effects on invasion, migration, nutrition and particularly survival of the parasite in the host tissues.

Cites

A COMPARISON BETWEEN THE EFFECTS OF ALBENDAZOLE AND MEBEN DAZOLE ON THE ENZYMATIC ACTIVITY OF EXCRETORY / SECRETORY PROD-UCTS OF ECHINOCOCCUS GRANULOSUS PROTOSCOLECES IN VITRO

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APA: Copy

SHRIFI, YOSEF, FARAHNAK, ALI, GOLESTANI, ABOLFAZLE, ESHRAGHIAN, MOHAMADREZA, FARIDI, ASHKAN, & MOLAEI RAD, MOHAMAD BAGHER. (2014). TRICLABENDAZOLE EFFECT ON PROTEASE ENZYME ACTIVITY IN THE EXCRETORY- SECRETORY PRODUCTS OF FASCIOLA HEPATICA IN VITRO. IRANIAN JOURNAL OF PARASITOLOGY, 9(1), 107-113. SID. https://sid.ir/paper/304027/en

Vancouver: Copy

SHRIFI YOSEF, FARAHNAK ALI, GOLESTANI ABOLFAZLE, ESHRAGHIAN MOHAMADREZA, FARIDI ASHKAN, MOLAEI RAD MOHAMAD BAGHER. TRICLABENDAZOLE EFFECT ON PROTEASE ENZYME ACTIVITY IN THE EXCRETORY- SECRETORY PRODUCTS OF FASCIOLA HEPATICA IN VITRO. IRANIAN JOURNAL OF PARASITOLOGY[Internet]. 2014;9(1):107-113. Available from: https://sid.ir/paper/304027/en

IEEE: Copy

YOSEF SHRIFI, ALI FARAHNAK, ABOLFAZLE GOLESTANI, MOHAMADREZA ESHRAGHIAN, ASHKAN FARIDI, and MOHAMAD BAGHER MOLAEI RAD, “TRICLABENDAZOLE EFFECT ON PROTEASE ENZYME ACTIVITY IN THE EXCRETORY- SECRETORY PRODUCTS OF FASCIOLA HEPATICA IN VITRO,” IRANIAN JOURNAL OF PARASITOLOGY, vol. 9, no. 1, pp. 107–113, 2014, [Online]. Available: https://sid.ir/paper/304027/en

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