مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

376
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

321
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

1

Information Journal Paper

Title

MOLECULAR IDENTIFICATION OF GIARDIA DUODENALIS ISOLATES FROM FARS PROVINCE, IRAN

Pages

  70-78

Abstract

 Background: GIARDIA DUODENALIS is one of the most common human intestinal pro-tozoan parasites worldwide and is endemic throughout the world with a vast range of mammalian hosts. The present study aimed to identify the PREVALENCE of G. duodenalis isolates and determine the most common of its assemblages in the patients referring to health centers and hospitals in Fars province, IRAN that will be subjected to further molecular investigation.Methods: We collected 1000 human fecal samples from health centers and hospitals in Shiraz, IRAN in a one year period from September 2009 to August 2010. MICROSCOPIC examination for the presence of G. duodenalis cysts and trophozoites was performed by direct wet mount before and after the concentration techniques. Extraction of DNA was performed by Phenol-Chloroform-Isoamylalcohol (PCI). G. duodenalis -positive specimens were analyzed by PCR. A fragment of the SSU-RDNA (292 bp) gene was amplified by PCR using the forward primer RH11 and the re-verse primer RH4. Genotyping was performed using sequence analysis of G. duodenalis glutamate dehydrogenase gene using primers GDHeF, GDHiF, and GDHiR.Results: The PREVALENCE of Giardia infection was 10.7% (107/1000) examined based on MICROSCOPIC examination. PCR identified 80% (40/50) of the samples as positive for G. duodenalis based on SSU-RDNA amplification on sucrose gradient samples. Besides, genotyping results indicated 32 isolates (80%) as assemblage AII and 8 isolates (20%) as assemblage BIII and BIV based on the DNA sequence analysis of the glutamate dehydrogenase locus of G. duodenalis.Conclusion: The findings of this study emphasize that IRAN (Fars Province) is a favorable area for giardiasis with an anthroponotic infection route.

Cites

References

  • No record.

    • Cite

    APA: Copy

    RAYANI, MOHAMMAD, ZASMY UNYAH, NGAH, & HATAM, GHOLAMREZA. (2014). MOLECULAR IDENTIFICATION OF GIARDIA DUODENALIS ISOLATES FROM FARS PROVINCE, IRAN. IRANIAN JOURNAL OF PARASITOLOGY, 9(1), 70-78. SID. https://sid.ir/paper/304037/en

    Vancouver: Copy

    RAYANI MOHAMMAD, ZASMY UNYAH NGAH, HATAM GHOLAMREZA. MOLECULAR IDENTIFICATION OF GIARDIA DUODENALIS ISOLATES FROM FARS PROVINCE, IRAN. IRANIAN JOURNAL OF PARASITOLOGY[Internet]. 2014;9(1):70-78. Available from: https://sid.ir/paper/304037/en

    IEEE: Copy

    MOHAMMAD RAYANI, NGAH ZASMY UNYAH, and GHOLAMREZA HATAM, “MOLECULAR IDENTIFICATION OF GIARDIA DUODENALIS ISOLATES FROM FARS PROVINCE, IRAN,” IRANIAN JOURNAL OF PARASITOLOGY, vol. 9, no. 1, pp. 70–78, 2014, [Online]. Available: https://sid.ir/paper/304037/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button