مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

MOLECULAR DETECTION AND IDENTIFICATION OF THEILERIA SPECIES BY PCR-RFLP METHOD IN SHEEP FROM AHVAZ, SOUTHERN IRAN

Pages

  99-106

Abstract

 Background: The present study was carried out to investigate the accurate status of ovine Theileria infection in SHEEP from Ahvaz and surrounding region, a tropical area southwest IRAN.Methods: A PCR-RFLP method based on 18S ribosomal RNA gene was designed which could detect and differentiate Theileria and Babesia spp. and also differentiate main Theileria species in SHEEP at the same time.119 SHEEP blood samples were collected from Ahvaz and surroundings.Results: Microscopic examination of blood smears revealed 69.7% (83/119) infection with Theileria spp. Of the total samples subjected to PCR, 89% (106/119) were found to be positive, all of which were identified as Theileria by RFLP analysis using enzyme Hind II. In enzymatic digestion of PCR products by Vsp I, 91.5% (97/106) of Theileria positive samples were identified as T. ovis while mixed Theileria infections were found in 9 samples. The samples with mixed infections were analyzed with an additional nested PCR-RFLP method, by HpaII enzyme digestion.3 samples with T. lestoquardi infection, 1 sample with T. ovis and T. annulata, 1 sample with T. lestoquardi and T. annulata, and 4 samples with T. ovis, T. lestoquardi and T. annulata mixed infections were detected.Conclusion: Ovine theileriosis caused by T. ovis is highly prevalent in southwest IRAN while T. lestoquardi and T. annulata infection can be detected in a lesser propor-tion of SHEEP in this region. The new PCR-RFLP method that was designed in this study, can serve as a beneficial diagnostic tool, especially in T. ovis prevalent regions.

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    APA: Copy

    JALALI, SEYEDEH MISSAGH, KHAKI, ZOHREH, KAZEMI, BAHRAM, RAHBARI, SADEGH, BANDEHPOUR, MOJGAN, & YASINI, SEYEDEH PARASTOO. (2014). MOLECULAR DETECTION AND IDENTIFICATION OF THEILERIA SPECIES BY PCR-RFLP METHOD IN SHEEP FROM AHVAZ, SOUTHERN IRAN. IRANIAN JOURNAL OF PARASITOLOGY, 9(1), 99-106. SID. https://sid.ir/paper/304045/en

    Vancouver: Copy

    JALALI SEYEDEH MISSAGH, KHAKI ZOHREH, KAZEMI BAHRAM, RAHBARI SADEGH, BANDEHPOUR MOJGAN, YASINI SEYEDEH PARASTOO. MOLECULAR DETECTION AND IDENTIFICATION OF THEILERIA SPECIES BY PCR-RFLP METHOD IN SHEEP FROM AHVAZ, SOUTHERN IRAN. IRANIAN JOURNAL OF PARASITOLOGY[Internet]. 2014;9(1):99-106. Available from: https://sid.ir/paper/304045/en

    IEEE: Copy

    SEYEDEH MISSAGH JALALI, ZOHREH KHAKI, BAHRAM KAZEMI, SADEGH RAHBARI, MOJGAN BANDEHPOUR, and SEYEDEH PARASTOO YASINI, “MOLECULAR DETECTION AND IDENTIFICATION OF THEILERIA SPECIES BY PCR-RFLP METHOD IN SHEEP FROM AHVAZ, SOUTHERN IRAN,” IRANIAN JOURNAL OF PARASITOLOGY, vol. 9, no. 1, pp. 99–106, 2014, [Online]. Available: https://sid.ir/paper/304045/en

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