FUNCTIONALLY EXPRESSION OF METALLOPROTEINASE IN TAENIA SOLIUM METACESTODE AND ITS EVALUATION FOR SERODIAGNOSIS OF CYSTICERCOSIS

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ZHANG YING; BAE YOUNG AN; ZONG HONG YING; KONG YOON; CAI GUO BIN

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Journal: IRANIAN JOURNAL OF PARASITOLOGY Year:2016 | Volume:11 | Issue:1 Page(s): 35-45

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Title

FUNCTIONALLY EXPRESSION OF METALLOPROTEINASE IN TAENIA SOLIUM METACESTODE AND ITS EVALUATION FOR SERODIAGNOSIS OF CYSTICERCOSIS

Author(s)

Keywords

TAENIA SOLIUM

METALLOPROTEINASE

HOST SUBSTRATE

SERODIAGNOSIS

CYSTICERCOSIS

Abstract

Background: Parasite proteases have important roles in cleavage of host proteins during the invasion of host tissues and participate in the parasite’s evasion from the host’s immune response. The aim of the present study was to estimate a metallo-proteinase properties of TAENIA SOLIUM metacestode (TsMP) during host-parasite interactions, and evaluate its potential as a serodiagnostic antigen for CYSTICERCOSIS.Methods: The cDNA coding for the mature catalytic domain of TsMP was cloned into pGEX-6P-1 expression vector. A recombinant glutathione S-transferase and TsMP fusion protein was induced. After refolding and purification, enzymatic properties of the recombinant METALLOPROTEINASE were observed. Immunoblot assay was processed to evaluate its potential as a serodiagnostic antigen for CYSTICERCOSIS.Results: The recombinant TsMP protein showed proteolytic activity, which pre-ferred host extracellular matrix proteins such as collagen and fibronectin as degradable substrates. In immunoblot assay, 87.5% of sera from patients with CYSTICERCOSIS showed strong reactivity. In sera from patients with other parasitic infections and from normal controls, it showed high specificity.Conclusions: TsMP might be involved in the processing of numerous host pro-teins and play an important role in the parasite life cycle. A single recombinant TsMP antigen could have a potential value for SERODIAGNOSIS of CYSTICERCOSIS.

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APA: Copy

ZHANG, YING, BAE, YOUNG AN, ZONG, HONG YING, KONG, YOON, & CAI, GUO BIN. (2016). FUNCTIONALLY EXPRESSION OF METALLOPROTEINASE IN TAENIA SOLIUM METACESTODE AND ITS EVALUATION FOR SERODIAGNOSIS OF CYSTICERCOSIS. IRANIAN JOURNAL OF PARASITOLOGY, 11(1), 35-45. SID. https://sid.ir/paper/304066/en

Vancouver: Copy

ZHANG YING, BAE YOUNG AN, ZONG HONG YING, KONG YOON, CAI GUO BIN. FUNCTIONALLY EXPRESSION OF METALLOPROTEINASE IN TAENIA SOLIUM METACESTODE AND ITS EVALUATION FOR SERODIAGNOSIS OF CYSTICERCOSIS. IRANIAN JOURNAL OF PARASITOLOGY[Internet]. 2016;11(1):35-45. Available from: https://sid.ir/paper/304066/en

IEEE: Copy

YING ZHANG, YOUNG AN BAE, HONG YING ZONG, YOON KONG, and GUO BIN CAI, “FUNCTIONALLY EXPRESSION OF METALLOPROTEINASE IN TAENIA SOLIUM METACESTODE AND ITS EVALUATION FOR SERODIAGNOSIS OF CYSTICERCOSIS,” IRANIAN JOURNAL OF PARASITOLOGY, vol. 11, no. 1, pp. 35–45, 2016, [Online]. Available: https://sid.ir/paper/304066/en

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