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Information Journal Paper

Title

Comparative Study of Wheatley’ s Trichrome Stain and In-vitro Culture against PCR Assay for the Diagnosis of Blastocystis sp. in Stool Samples (Short Communication)

Pages

  127-136

Abstract

 Background: This study evaluated the performance of routine permanent stain and cultivation method in comparison with polymerase chain reaction assay as the reference technique to detect Blastocystis sp. Methods: A cross-sectional study was conducted among aboriginal populations that reside in Pahang, Peninsular Malaysia in Feb to Mar 2015. A total of 359 stool samples were examined using Wheatley’ s Trichrome stain, in-vitro cultivation in Jones’ medium and PCR assay. Positive amplicons were subjected to sequencing and phylogenetic analysis. Results: Fifty-six (15. 6%) samples were detected positive with Blastocystis sp. by Wheatley’ s Trichrome stain and 73 (20. 3%) by In-vitro culture, while PCR assay detected 71 (19. 8%) positive samples. Detection rate of Blastocystis sp. was highest in combination of microscopic techniques (27. 9%). The sensitivity and specificity of Wheatley’ s Trichrome staining and In-vitro culture techniques compared to PCR assay were 49. 3% (95% CI: 37. 2-61. 4) and 92. 7% (95% CI: 89. 1-95. 4) and 39. 4% (95% CI: 28. 0-51. 8) and 84. 4% (95% CI: 79. 7-88. 4), respectively. However, the sensitivity [60. 6% (95% CI: 48. 3-71. 9)] of the method increased when both microscopic techniques were performed together. False negative results produced by microscopic techniques were associated with subtype 3. The agreement between Wheatley’ s Trichrome stain, In-vitro culture and combination of microscopic techniques with PCR assay were statistically significant by Kappa statistics (Wheatley’ s Trichrome stain: K = 0. 456, P<0. 001; In-vitro culture: K = 0. 236, P<0. 001 and combination techniques: K = 0. 353, P<0. 001). Conclusion: The combination of microscopic technique is highly recommended to be used as a screening method for the diagnosis of Blastocystis infection either for clinical or epidemiological study to ensure better and accurate diagnosis.

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    APA: Copy

    MOHAMMAD, Nabilah Amelia, MASTUKI, Mohd Fahmi, M. AL MEKHLAFI, Hesham, MOKTAR, Norhayati, & ANUAR, Tengku Shahrul. (2018). Comparative Study of Wheatley’ s Trichrome Stain and In-vitro Culture against PCR Assay for the Diagnosis of Blastocystis sp. in Stool Samples (Short Communication). IRANIAN JOURNAL OF PARASITOLOGY, 13(1), 127-136. SID. https://sid.ir/paper/304128/en

    Vancouver: Copy

    MOHAMMAD Nabilah Amelia, MASTUKI Mohd Fahmi, M. AL MEKHLAFI Hesham, MOKTAR Norhayati, ANUAR Tengku Shahrul. Comparative Study of Wheatley’ s Trichrome Stain and In-vitro Culture against PCR Assay for the Diagnosis of Blastocystis sp. in Stool Samples (Short Communication). IRANIAN JOURNAL OF PARASITOLOGY[Internet]. 2018;13(1):127-136. Available from: https://sid.ir/paper/304128/en

    IEEE: Copy

    Nabilah Amelia MOHAMMAD, Mohd Fahmi MASTUKI, Hesham M. AL MEKHLAFI, Norhayati MOKTAR, and Tengku Shahrul ANUAR, “Comparative Study of Wheatley’ s Trichrome Stain and In-vitro Culture against PCR Assay for the Diagnosis of Blastocystis sp. in Stool Samples (Short Communication),” IRANIAN JOURNAL OF PARASITOLOGY, vol. 13, no. 1, pp. 127–136, 2018, [Online]. Available: https://sid.ir/paper/304128/en

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