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Information Journal Paper

Title

Comparison of Three PCR-based Methods for Simplicity and Cost Effectiveness Identification of Cutaneous Leishmaniasis Due to Leishmania tropica

Pages

  215-223

Abstract

 Background: To compare three molecular methods, PCR-RFLP for internal transcribed spacer, PCR sequencing and high resolution melting analysis shown reliable sensitivity and specificity for detecting Leishmania tropica as a model for Cutaneous leishmaniasis (CL) as the perspective overview for scientific and economic approaches. Methods: This study was carried out between 2015 and 2016 in Leishmaniasis Research Center in Kerman University of Medical Sciences, Kerman, Iran. The positives smears (n=50) were obtained from patients referred from the health clinics in a major anthroponotic CL (ACL) focus, southeastern Iran. Only smear preparations with the same grade were selected according to the method described by the WHO for future PCR assays. Results: All three molecular methods had capability to identify positive samples at species level with the same specificity and sensitivity. However, these techniques were different in simplicity, consuming time, and cost effectiveness. Although additional enzymatic process in PCR-RFLP provided good resolution to find Leishmania species but this would cause time and cost increases. Conclusion: HRM (high resolution melting) is a relatively new technique that allows direct characterization of PCR amplicons in a closed system with more simplicity, cost effectiveness and time-consuming compared with other PCR-based assays for epidemiological or clinical identification purposes.

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    • Cite

    APA: Copy

    MOHAMMADI, MOHAMMAD ALI, BAMOROVAT, MEHDI, FASIHI HARANDI, MAJID, KARIMI, TAYYEBEH, SHARIFI, IRAJ, & AFLATOONIAN, MOHAMMAD REZA. (2017). Comparison of Three PCR-based Methods for Simplicity and Cost Effectiveness Identification of Cutaneous Leishmaniasis Due to Leishmania tropica. IRANIAN JOURNAL OF PARASITOLOGY, 12(2), 215-223. SID. https://sid.ir/paper/304181/en

    Vancouver: Copy

    MOHAMMADI MOHAMMAD ALI, BAMOROVAT MEHDI, FASIHI HARANDI MAJID, KARIMI TAYYEBEH, SHARIFI IRAJ, AFLATOONIAN MOHAMMAD REZA. Comparison of Three PCR-based Methods for Simplicity and Cost Effectiveness Identification of Cutaneous Leishmaniasis Due to Leishmania tropica. IRANIAN JOURNAL OF PARASITOLOGY[Internet]. 2017;12(2):215-223. Available from: https://sid.ir/paper/304181/en

    IEEE: Copy

    MOHAMMAD ALI MOHAMMADI, MEHDI BAMOROVAT, MAJID FASIHI HARANDI, TAYYEBEH KARIMI, IRAJ SHARIFI, and MOHAMMAD REZA AFLATOONIAN, “Comparison of Three PCR-based Methods for Simplicity and Cost Effectiveness Identification of Cutaneous Leishmaniasis Due to Leishmania tropica,” IRANIAN JOURNAL OF PARASITOLOGY, vol. 12, no. 2, pp. 215–223, 2017, [Online]. Available: https://sid.ir/paper/304181/en

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