مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

269
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

138
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

The Impact of Different Copro-preservation Conditions on Molecular Detection of Cryptosporidium Species

Pages

  274-283

Abstract

 Background: We aimed to evaluate different copro-preservation conditions along the duration of one month for a better outcome of Molecular diagnosis of Cryptosporidium species. Methods: Ten samples out of 380 fresh stool samples collected from patients with Diarrhea proved positive after direct examination, concentration, staining and confirmed by immunochromatographic test. The study was conducted at the Diagnostic and Research Unit of Parasitic diseases, Faculty of Medicine, Ain Shams University at the time interval from July 2014 to December 2015. Each stool sample was preserved in five different conditions; freezing at-20 º C, 70% ethyl alcohol, 10% formalin, 2. 5% potassium dichromate (K dichromate) at 4 º C and 2. 5% K dichromate at room temperature (RT). Then DNA extraction and Nested PCR, with Cryptosporidium oocyst wall protein (COWP) gene were done from each sample at zero time (fresh specimens) as a standard for comparison with the preservation conditions at 10, 20 and 30 d. Results: Sensitivity of studied preservative conditions along the whole study duration showed best outcome from freezing at-20 ° C (80%) then K dichromate (4 ° C) (73. 3%) followed by K dichromate (RT) (66. 7%), then alcohol (33. 3%), while formalin was the worst (0%) with a highly significant comparative outcome between the different conditions. Along the three extraction intervals, K dichromate (RT), unlike all the rest of conditions lacks the consistent preservative action. Conclusion: Our study highlights freezing at-20 º C to be the most suitable condition for preservation followed by K dichromate at 4 ° C, K dichromate at RT, then 70% ethyl alcohol. Formalin (10%) is better to be avoided.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    ABDELSALAM, Iman Moawad, SARHAN, Rania Mohammad, & HANAFY, Marmar Ahmed. (2017). The Impact of Different Copro-preservation Conditions on Molecular Detection of Cryptosporidium Species. IRANIAN JOURNAL OF PARASITOLOGY, 12(2), 274-283. SID. https://sid.ir/paper/304184/en

    Vancouver: Copy

    ABDELSALAM Iman Moawad, SARHAN Rania Mohammad, HANAFY Marmar Ahmed. The Impact of Different Copro-preservation Conditions on Molecular Detection of Cryptosporidium Species. IRANIAN JOURNAL OF PARASITOLOGY[Internet]. 2017;12(2):274-283. Available from: https://sid.ir/paper/304184/en

    IEEE: Copy

    Iman Moawad ABDELSALAM, Rania Mohammad SARHAN, and Marmar Ahmed HANAFY, “The Impact of Different Copro-preservation Conditions on Molecular Detection of Cryptosporidium Species,” IRANIAN JOURNAL OF PARASITOLOGY, vol. 12, no. 2, pp. 274–283, 2017, [Online]. Available: https://sid.ir/paper/304184/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button