مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

216
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

104
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

Holothuria leucospilota Extract Induces Apoptosis in Leishmania major Promastigotes

Pages

  339-349

Abstract

 Background: The present study aimed to survey antileishmanial activity of methanolic Holothuria leucospilota extract against Leishmania major promastigotes in vitro. Methods: Promastigotes were cultured in RPMI 1640 and after reaching the stationary phase, the study was conducted with different concentrations of the extract. Afterwards, MTT colorimetric assay for the obtaining of 50% inhibitory concentration (IC50) was utilized. Furthermore, in order to determine the possible induction of Apoptosis in L. major promastigotes, flow cytometry and DNA Fragmentation methods were employed using annexin-V FLUOS staining kit and DNA ladder kit, respectively. Results: The IC50 value of H. leucospilota extract at three time points of 24, 48, and 72 h was estimated 2000, 300 and 85 μ g/ml, respectively. In addition, the extract revealed a dose and time-dependent antileishmanial activity. Furthermore, various characteristics of Apoptosis appeared after L. major promastigotes treatment, which included cell shrinkage, formation of apoptotic bodies, blebbing of the cell membrane, and externalization of phosphatidylserine, although no laddering pattern was observed. Conclusion: The methanolic extract of H. leucospilota possesses lethal effect on L. major promastigotes and induces the Apoptosis in parasites. Further studies are required to address the Apoptosis mechanism in vivo.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    FOROUTAN RAD, MASOUD, KHADEMVATAN, SHAHRAM, SAKI, JASEM, & HASHEMITABAR, MAHMOUD. (2016). Holothuria leucospilota Extract Induces Apoptosis in Leishmania major Promastigotes. IRANIAN JOURNAL OF PARASITOLOGY, 11(3), 339-349. SID. https://sid.ir/paper/304198/en

    Vancouver: Copy

    FOROUTAN RAD MASOUD, KHADEMVATAN SHAHRAM, SAKI JASEM, HASHEMITABAR MAHMOUD. Holothuria leucospilota Extract Induces Apoptosis in Leishmania major Promastigotes. IRANIAN JOURNAL OF PARASITOLOGY[Internet]. 2016;11(3):339-349. Available from: https://sid.ir/paper/304198/en

    IEEE: Copy

    MASOUD FOROUTAN RAD, SHAHRAM KHADEMVATAN, JASEM SAKI, and MAHMOUD HASHEMITABAR, “Holothuria leucospilota Extract Induces Apoptosis in Leishmania major Promastigotes,” IRANIAN JOURNAL OF PARASITOLOGY, vol. 11, no. 3, pp. 339–349, 2016, [Online]. Available: https://sid.ir/paper/304198/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button