COMBINATION OF IN VIVO CRYPTORCHID TESTIS AND IN VITRO CO-CULTURE SYSTEM TO OBTAIN HIGH PURIFICATION AND PROLIFERATION OF MOUSE SPERMATOGONIAL STEM CELLS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ABSALAN F.; MOVAHEDIN M.; MOULA SEYED JAVAD

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: International Journal of Fertility & Sterility Year:2008 | Volume:2 | Issue:3 Page(s): 115-120

Download Full-Text

View:

447

Download:

202

Cites:

Information Journal Paper

Title

COMBINATION OF IN VIVO CRYPTORCHID TESTIS AND IN VITRO CO-CULTURE SYSTEM TO OBTAIN HIGH PURIFICATION AND PROLIFERATION OF MOUSE SPERMATOGONIAL STEM CELLS

Author(s)

ABSALAN F. | MOVAHEDIN M. | MOULA SEYED JAVAD | Issue Writer Certificate

Keywords

CO-CULTURE

COLONIZATION

SPERMATOGONIAL

CRYPTORCHIDISM

Abstract

Background: The present study was designed to evaluate the survival and proliferation of SPERMATOGONIAL stem cells from cryptorchid mouse testis in CO-CULTURE system over a 3 weeks period. Materials and Methods: Sertoli and SPERMATOGONIAL cells were isolated from bilateral cryptorchid mouse model testes. Isolated SPERMATOGONIAL cells were CO-CULTUREd with Sertoli cells in minimal essential medium (a-MEM) supplemented with 10% fetal calf serum (FCS) for three weeks. The identity of the cells was confirmed through immunocytochemistry against Oct-4 and Vimentin. Results: Best results were achieved from the CO-CULTURE system spermatogonia which continued to proliferate, and eventually, type A spermatogonia colonies were found. Most of the cells in these colonies were Oct-4 positive. Conclusion: Bilateral cryptorchid surgery model is a good model for enrichment of SPERMATOGONIAL stem cells (SSCs). These cells can be used for molecular characterization, genetic manipulation and restoration of male fertility.

Cites

No record.

References

Cite

APA: Copy

ABSALAN, F., MOVAHEDIN, M., & MOULA, SEYED JAVAD. (2008). COMBINATION OF IN VIVO CRYPTORCHID TESTIS AND IN VITRO CO-CULTURE SYSTEM TO OBTAIN HIGH PURIFICATION AND PROLIFERATION OF MOUSE SPERMATOGONIAL STEM CELLS. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, 2(3), 115-120. SID. https://sid.ir/paper/304451/en

Vancouver: Copy

ABSALAN F., MOVAHEDIN M., MOULA SEYED JAVAD. COMBINATION OF IN VIVO CRYPTORCHID TESTIS AND IN VITRO CO-CULTURE SYSTEM TO OBTAIN HIGH PURIFICATION AND PROLIFERATION OF MOUSE SPERMATOGONIAL STEM CELLS. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY[Internet]. 2008;2(3):115-120. Available from: https://sid.ir/paper/304451/en

IEEE: Copy

F. ABSALAN, M. MOVAHEDIN, and SEYED JAVAD MOULA, “COMBINATION OF IN VIVO CRYPTORCHID TESTIS AND IN VITRO CO-CULTURE SYSTEM TO OBTAIN HIGH PURIFICATION AND PROLIFERATION OF MOUSE SPERMATOGONIAL STEM CELLS,” INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, vol. 2, no. 3, pp. 115–120, 2008, [Online]. Available: https://sid.ir/paper/304451/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops