EFFECTS OF TREATMENT WITH BONE MORPHOGENETIC PROTEIN 4 AND CO-CULTURE ON EXPRESSION OF PIWIL2 GENE IN MOUSE DIFFERENTIATED EMBRYONIC STEM CELLS

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MAKOULATI Z.; MOVAHEDIN MANSOUREH; FOROUZANDEH MOGHADAM M.

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Journal: International Journal of Fertility & Sterility Year:2009 | Volume:3 | Issue:2 Page(s): 78-83

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Title

EFFECTS OF TREATMENT WITH BONE MORPHOGENETIC PROTEIN 4 AND CO-CULTURE ON EXPRESSION OF PIWIL2 GENE IN MOUSE DIFFERENTIATED EMBRYONIC STEM CELLS

Author(s)

MAKOULATI Z. | MOVAHEDIN MANSOUREH | FOROUZANDEH MOGHADAM M. | Issue Writer Certificate

Keywords

EMBRYONIC STEM CELL

GERM CELL

BMP4

PIWIL2

CO-CULTURE

Abstract

Background: Specific growth factors and feeder layers seem to have important roles in vitro EMBRYONIC STEM CELLs (ESCs) differentiation. In this study, the effects of bone morphogenetic protein 4 (BMP4) and mouse embryonic fibroblasts (MEFs) CO-CULTURE system on GERM CELL differentiation from mouse ESCs were studied.Materials and Methods: Cell suspension was prepared from one-day-old embryoid body (EB) and cultured for four days in DMEM medium containing 20% fetal bovine serum (FBS) in the following groups: simple culture (SC), simple culture with BMP4 (SCB), CO-CULTURE (CO-C) and CO-CULTURE with BMP4 (CO-CB). Expression of piwi-like homolog 2 (Piwil2), the GERM CELL-specific gene, was evaluated in the different study groups by using quantitative real time polymerase chain reaction (RT-PCR). Testis was used as a positive control.Results: The maximum and minimum PIWIL2 expression was observed in SC and SCB groups, respectively. A significant difference was observed in PIWIL2 expression between SCB and other study groups (p<0.05).Conclusion: The findings of this study showed that neither the addition of BMP4 in culture medium nor the use of MEFs as a feeder layer have a positive effect on late GERM CELL induction from mouse ESCs.

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APA: Copy

MAKOULATI, Z., MOVAHEDIN, MANSOUREH, & FOROUZANDEH MOGHADAM, M.. (2009). EFFECTS OF TREATMENT WITH BONE MORPHOGENETIC PROTEIN 4 AND CO-CULTURE ON EXPRESSION OF PIWIL2 GENE IN MOUSE DIFFERENTIATED EMBRYONIC STEM CELLS. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, 3(2), 78-83. SID. https://sid.ir/paper/304473/en

Vancouver: Copy

MAKOULATI Z., MOVAHEDIN MANSOUREH, FOROUZANDEH MOGHADAM M.. EFFECTS OF TREATMENT WITH BONE MORPHOGENETIC PROTEIN 4 AND CO-CULTURE ON EXPRESSION OF PIWIL2 GENE IN MOUSE DIFFERENTIATED EMBRYONIC STEM CELLS. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY[Internet]. 2009;3(2):78-83. Available from: https://sid.ir/paper/304473/en

IEEE: Copy

Z. MAKOULATI, MANSOUREH MOVAHEDIN, and M. FOROUZANDEH MOGHADAM, “EFFECTS OF TREATMENT WITH BONE MORPHOGENETIC PROTEIN 4 AND CO-CULTURE ON EXPRESSION OF PIWIL2 GENE IN MOUSE DIFFERENTIATED EMBRYONIC STEM CELLS,” INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, vol. 3, no. 2, pp. 78–83, 2009, [Online]. Available: https://sid.ir/paper/304473/en

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