EFFECT OF CULTURE SYSTEM ON DEVELOPMENTAL COMPETENCE, CRYOSURVIVAL AND DNA-FRAGMENTATION OF IN VITRO BOVINE BLASTOCYSTS

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HAJIAN MAHDI; HOSSEINI SEYED MORTEZA; ASGARI VAJIHEH; OSTADHOOSSEINI SOMAYYEH; FOROUZANFAR MOHSEN; NASR ESFAHANI MOHAMMAD HOSSEIN

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Journal: International Journal of Fertility & Sterility Year:2011 | Volume:5 | Issue:1 Page(s): 21-26

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Title

EFFECT OF CULTURE SYSTEM ON DEVELOPMENTAL COMPETENCE, CRYOSURVIVAL AND DNA-FRAGMENTATION OF IN VITRO BOVINE BLASTOCYSTS

Author(s)

Keywords

BLASTOCYST

VITRIFICATION

CRYOPERSERVATION

EMBRYO DEVELOPMENT

CO-CULTURE

Abstract

Background: This study investigated the effect of two in vitro embryo culture systems (co-culture system versus cell-free sequential-media) on developmental competence, cryosurvival and DNAfragmentation of in vitro developed bovine BLASTOCYSTs.Materials and Methods: Bovine presumptive zygotes were cultured in Ménézo's B2 (B2) plus vero-cells or sequential synthetic oviductal fluid (SOF) for eight days. Subsequently, half of the expanded BLASTOCYSTs developed in both groups were vitrified, warmed within 30 minutes and postwarming embryos along with their corresponding non-vitrified embryos were cultured for two additional days in the same medium used before VITRIFICATION. EMBRYO DEVELOPMENT, cryosurvival and apoptosis were compared between the groups.Results: For non-vitrified embryos, culture in SOF significantly promoted the potency of embryos to develop into BLASTOCYSTs compared with the CO-CULTURE system. The difference in post VITRIFICATION survival rate of SOF BLASTOCYSTs (83.3%) was insignificant compared with CO-CULTURE (84.3%).However, while total cell number of warmed BLASTOCYSTs in the CO-CULTURE system was significantly higher in the CO-CULTURE versus the sequential system (215.4 vs.170.4), the quality of survived embryos in terms of hatching ability and apoptosis was adversely affected by CO-CULTURE compared with SOF (65.0% vs.74.3%, and 13.5% vs.10.0%, respectively; p<0.05).Conclusion: Although CO-CULTURE system may increase the viability of embryos following cryopreservation, the potency and dynamics of BLASTOCYST formation significantly increased with sequential media compared to the CO-CULTURE system which can compensate for the lower efficiency of sequential media for VITRIFICATION/warming purposes.

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APA: Copy

HAJIAN, MAHDI, HOSSEINI, SEYED MORTEZA, ASGARI, VAJIHEH, OSTADHOOSSEINI, SOMAYYEH, FOROUZANFAR, MOHSEN, & NASR ESFAHANI, MOHAMMAD HOSSEIN. (2011). EFFECT OF CULTURE SYSTEM ON DEVELOPMENTAL COMPETENCE, CRYOSURVIVAL AND DNA-FRAGMENTATION OF IN VITRO BOVINE BLASTOCYSTS. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, 5(1), 21-26. SID. https://sid.ir/paper/304732/en

Vancouver: Copy

HAJIAN MAHDI, HOSSEINI SEYED MORTEZA, ASGARI VAJIHEH, OSTADHOOSSEINI SOMAYYEH, FOROUZANFAR MOHSEN, NASR ESFAHANI MOHAMMAD HOSSEIN. EFFECT OF CULTURE SYSTEM ON DEVELOPMENTAL COMPETENCE, CRYOSURVIVAL AND DNA-FRAGMENTATION OF IN VITRO BOVINE BLASTOCYSTS. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY[Internet]. 2011;5(1):21-26. Available from: https://sid.ir/paper/304732/en

IEEE: Copy

MAHDI HAJIAN, SEYED MORTEZA HOSSEINI, VAJIHEH ASGARI, SOMAYYEH OSTADHOOSSEINI, MOHSEN FOROUZANFAR, and MOHAMMAD HOSSEIN NASR ESFAHANI, “EFFECT OF CULTURE SYSTEM ON DEVELOPMENTAL COMPETENCE, CRYOSURVIVAL AND DNA-FRAGMENTATION OF IN VITRO BOVINE BLASTOCYSTS,” INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, vol. 5, no. 1, pp. 21–26, 2011, [Online]. Available: https://sid.ir/paper/304732/en

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