THE PRODUCTION OF MONOCLONAL ANTIBODY AGAINST A60 FROM MYCOBACTERIUM BOVIS BCG

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BAKHTIARI SALAR; TAGHIKHANI M.; KHABIRI A.R.; MOUKHAH R.; MOHAMMADNEZHAD AROUGH J.

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Journal: DANESHVAR MEDICINE Year:2007 | Volume:14 | Issue:66 Page(s): 1-6

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Title

THE PRODUCTION OF MONOCLONAL ANTIBODY AGAINST A60 FROM MYCOBACTERIUM BOVIS BCG

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Keywords

MONOCLONAL ANTIBODYQ2

MYCOBACTERIUM BOVIS BCGQ3

HYBRIDOMAQ4

A60 ANTIGENQ3

Abstract

Introduction: MONOCLONAL ANTIBODY against A60 ANTIGEN of MYCOBACTERIUM BOVIS BCG has many applications. The most important of these include: designation of ELlSA-based rapid diagnostic kit for diagnosis of tuberculosis and chemotherapy of cancer. One of the common methods in generation of MONOCLONAL ANTIBODY is the hybridization of splenocytes of immunized mice with myeloma cells.Materials & Method: In this investigation Balb/c mice were immunized by several injections of BCG and A60 ANTIGEN. Antibody titer was measured after each injection. The splenocytes of immunized mice and SP2/0 myeloma cells were fused with 50% polyethylene glycol (PEG) as fusing agent. One fusion was performed and 62 clones resulted. Then, HYBRIDOMA cells were selected by HAT medium. Screening of HYBRIDOMA supernatants was performed by ELlSA test on supernatant of all resulted HYBRIDOMA clones. Limiting dilution was performed on two clones (MB1D5, MB3F8) which had higher absorbance in ELlSA test and the resulted subclones had multiplied.

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APA: Copy

BAKHTIARI, SALAR, MOUKHAH, R., MOHAMMADNEZHAD AROUGH, J., TAGHIKHANI, M., & KHABIRI, A.R.. (2007). THE PRODUCTION OF MONOCLONAL ANTIBODY AGAINST A60 FROM MYCOBACTERIUM BOVIS BCG. DANESHVAR MEDICINE, 14(66), 1-6. SID. https://sid.ir/paper/30507/en

Vancouver: Copy

BAKHTIARI SALAR, MOUKHAH R., MOHAMMADNEZHAD AROUGH J., TAGHIKHANI M., KHABIRI A.R.. THE PRODUCTION OF MONOCLONAL ANTIBODY AGAINST A60 FROM MYCOBACTERIUM BOVIS BCG. DANESHVAR MEDICINE[Internet]. 2007;14(66):1-6. Available from: https://sid.ir/paper/30507/en

IEEE: Copy

SALAR BAKHTIARI, R. MOUKHAH, J. MOHAMMADNEZHAD AROUGH, M. TAGHIKHANI, and A.R. KHABIRI, “THE PRODUCTION OF MONOCLONAL ANTIBODY AGAINST A60 FROM MYCOBACTERIUM BOVIS BCG,” DANESHVAR MEDICINE, vol. 14, no. 66, pp. 1–6, 2007, [Online]. Available: https://sid.ir/paper/30507/en

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