BENEFICIAL EFFECTS OF NITRIC OXIDE INDUCED MILD OXIDATIVE STRESS ON POST-THAWED BULL SEMEN QUALITY

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SHARAFI MOHSEN; ZHANDI MAHDI; SHAHVERDI ABDOLHOSSEIN; SHAKERI MALAK

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Journal: International Journal of Fertility & Sterility Year:2015 | Volume:9 | Issue:2 Page(s): 230-237

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Information Journal Paper

Title

BENEFICIAL EFFECTS OF NITRIC OXIDE INDUCED MILD OXIDATIVE STRESS ON POST-THAWED BULL SEMEN QUALITY

Author(s)

SHARAFI MOHSEN | ZHANDI MAHDI | SHAHVERDI ABDOLHOSSEIN | SHAKERI MALAK | Issue Writer Certificate

Keywords

BULL

CRYOPRESERVATION

NITRIC OXIDE

OXIDATIVE STRESS

SPERM

Abstract

Background: CRYOPRESERVATION of semen requires optimized conditions to minimize the harmful effects of various stresses. The main approach for protection of SPERM against stress is based on the use of antioxidants and cryoprotectants, which are described as defensive methods. Recently, the application of controlled mild stressors has been described for activation of a temporary response in oocyte, embryo and somatic cells. In this study a sub-lethal OXIDATIVE STRESS induced by precise concentrations of NITRIC OXIDE (NO) has been evaluated for SPERM during CRYOPRESERVATION.Materials and Methods: In this experimental study, we used different concentrations of NO [0 mM (NO-0), 0.01 mM (NO-0.01), 0.1 mM (NO-0.1), 1 mM (NO-1), 10 mM (NO-10) and 100 mM (NO-100)] during CRYOPRESERVATION of BULL semen. Their effects on post-thawed SPERM quality that included motility and velocity parameters, plasma membrane functionality, acrosome integrity, apoptosis status, mitochondrial activity and lipid peroxidation after freezing-thawing were investigated.Results: Exposure of SPERM before freezing to NO-1 significantly increased total motility (88.4±2.8%), progressive motility (50.4±3.2%) and average path velocity (VAP, 53.8±3.1 μm/s) compared to other extenders. In addition, NO-1 significantly increased plasma membrane functionality (89.3±2.9%) compared to NO-0 (75.3±2.9%), NO-0.01 (78.3±2.9%), NO-0.1 (76.4±2.9%), NO-10 (64±2.9%) and NO-100 (42±2.9%). SPERM exposed to NO-1 produced the highest percentage of viable (85.6±2.3%) and the lowest percentage of apoptotic (10.8±2.4%) SPERMatozoa compared to the other extenders. Also, NO-100 resulted in a higher percentage of dead SPERMatozoa (27.1±2.7%) compared to the other extenders. In terms of mitochondrial activity, there was no significant difference among NO-0 (53.4±3.2), NO-0.01 (52.1±3.2), NO-0.1 (50.8±3.2) and NO-1 (53.1±3.2). For acrosome integrity, no significant different was observed in SPERM exposed to different concentrations of NO.Conclusion: Induction of sub-lethal OXIDATIVE STRESS with 1 mM NO would be beneficial for CRYOPRESERVATION of BULL semen.

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APA: Copy

SHARAFI, MOHSEN, ZHANDI, MAHDI, SHAHVERDI, ABDOLHOSSEIN, & SHAKERI, MALAK. (2015). BENEFICIAL EFFECTS OF NITRIC OXIDE INDUCED MILD OXIDATIVE STRESS ON POST-THAWED BULL SEMEN QUALITY. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, 9(2), 230-237. SID. https://sid.ir/paper/305812/en

Vancouver: Copy

SHARAFI MOHSEN, ZHANDI MAHDI, SHAHVERDI ABDOLHOSSEIN, SHAKERI MALAK. BENEFICIAL EFFECTS OF NITRIC OXIDE INDUCED MILD OXIDATIVE STRESS ON POST-THAWED BULL SEMEN QUALITY. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY[Internet]. 2015;9(2):230-237. Available from: https://sid.ir/paper/305812/en

IEEE: Copy

MOHSEN SHARAFI, MAHDI ZHANDI, ABDOLHOSSEIN SHAHVERDI, and MALAK SHAKERI, “BENEFICIAL EFFECTS OF NITRIC OXIDE INDUCED MILD OXIDATIVE STRESS ON POST-THAWED BULL SEMEN QUALITY,” INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, vol. 9, no. 2, pp. 230–237, 2015, [Online]. Available: https://sid.ir/paper/305812/en

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