EX VIVO EXPANSION OF CORD BLOOD MONONUCLEAR CELLS BY UMBILICAL CORD BLOOD PLATELET LYSATE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MOHAMMAD MONIREH; EBRAHIMI MARZIEH; DANESHMAND FATEMEH; NOURI MASOUMEH; KHOSRAVANI PARDIS

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: DANESHVAR MEDICINE Year:2017 | Volume:24 | Issue:127 Page(s): 49-61

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

1,030

Download:

Cites:

Information Journal Paper

Title

EX VIVO EXPANSION OF CORD BLOOD MONONUCLEAR CELLS BY UMBILICAL CORD BLOOD PLATELET LYSATE

Author(s)

Keywords

PROLIFERATION AND DIFFERENTIATIONQ1

PLATELET EXTRACTS DERIVED FROM UMBILICAL CORD BLOODQ1

COLONY ASSAYQ1

Abstract

Background and Objective: In spite of promising results in treatment of blood diseases by umbilical cord blood cell transplantation, low number of derived stem cells has been a matter of concern. Since platelets extracts has been introduced as a potent mesenchymal stem cell culture supplement, the purpose of current study was to investigate the probable effects of umbilical cord blood platelet lysate (UCB-PL) in proliferation, survival and differentiation of cord blood mononuclear cells.Materials and Methods: The umbilical cord blood platelet lysate (UCB-PL) was added to cord blood mononuclear cells culture (1×10 6 /mL) in presence of FLT3L, TPO and SCF. The effective dose of UCB-PL, cell viability and differentiation of mononuclear cells were determined by trypan blue staining and COLONY ASSAY method, respectively. The number of mononuclear cells was measured by flowcytometric analysis of CD133+CD34+CD38- cells.Quantitative expression of CD 34 was performed by QRT-PCR method.Results: Combination of 5% of UCB-PL with mentioned cytokines yielded a 12-fold cell proliferation in comparison with control group. A higher concentration of UCB-PL showed cytotoxic effects. CD 34 also increased significantly in group of 5% UCB-PL treated cells. In this particular group, a significant increase of colony forming units granulocytes-erythroidmacrophages-megakaryocyte (CFU-GMMM) and granulocyte-macrophage (CFU-GM) were detected.Conclusion: Our results indicate that 5% UCB -PL can be used as a supplement in MNC cultured cells and in cord blood hematopoietic stem cells for cell therapy purposes.

Cites

No record.

References

No record.

Cite

APA: Copy

MOHAMMAD, MONIREH, EBRAHIMI, MARZIEH, DANESHMAND, FATEMEH, NOURI, MASOUMEH, & KHOSRAVANI, PARDIS. (2017). EX VIVO EXPANSION OF CORD BLOOD MONONUCLEAR CELLS BY UMBILICAL CORD BLOOD PLATELET LYSATE. DANESHVAR MEDICINE, 24(127 ), 49-61. SID. https://sid.ir/paper/30588/en

Vancouver: Copy

MOHAMMAD MONIREH, EBRAHIMI MARZIEH, DANESHMAND FATEMEH, NOURI MASOUMEH, KHOSRAVANI PARDIS. EX VIVO EXPANSION OF CORD BLOOD MONONUCLEAR CELLS BY UMBILICAL CORD BLOOD PLATELET LYSATE. DANESHVAR MEDICINE[Internet]. 2017;24(127 ):49-61. Available from: https://sid.ir/paper/30588/en

IEEE: Copy

MONIREH MOHAMMAD, MARZIEH EBRAHIMI, FATEMEH DANESHMAND, MASOUMEH NOURI, and PARDIS KHOSRAVANI, “EX VIVO EXPANSION OF CORD BLOOD MONONUCLEAR CELLS BY UMBILICAL CORD BLOOD PLATELET LYSATE,” DANESHVAR MEDICINE, vol. 24, no. 127 , pp. 49–61, 2017, [Online]. Available: https://sid.ir/paper/30588/en

Related Journal Papers