EX VIVO EXPANSION OF CORD BLOOD MONONUCLEAR CELLS BY UMBILICAL CORD BLOOD PLATELET LYSATE

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MOHAMMAD MONIREH; EBRAHIMI MARZIEH; DANESHMAND FATEMEH; NOURI MASOUMEH; KHOSRAVANI PARDIS

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Journal: Daneshvar Medicine: Basic and Clinical Research Journal Year:2017 | Volume:24 | Issue:127 Page(s): 49-61

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Title

EX VIVO EXPANSION OF CORD BLOOD MONONUCLEAR CELLS BY UMBILICAL CORD BLOOD PLATELET LYSATE

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Keywords

PROLIFERATION AND DIFFERENTIATIONQ1

PLATELET EXTRACTS DERIVED FROM UMBILICAL CORD BLOODQ1

COLONY ASSAYQ1

Abstract

Background and Objective: In spite of promising results in treatment of blood diseases by umbilical cord blood cell transplantation, low number of derived stem cells has been a matter of concern. Since platelets extracts has been introduced as a potent mesenchymal stem cell culture supplement, the purpose of current study was to investigate the probable effects of umbilical cord blood platelet lysate (UCB-PL) in proliferation, survival and differentiation of cord blood mononuclear cells.Materials and Methods: The umbilical cord blood platelet lysate (UCB-PL) was added to cord blood mononuclear cells culture (1×10 6 /mL) in presence of FLT3L, TPO and SCF. The effective dose of UCB-PL, cell viability and differentiation of mononuclear cells were determined by trypan blue staining and COLONY ASSAY method, respectively. The number of mononuclear cells was measured by flowcytometric analysis of CD133+CD34+CD38- cells.Quantitative expression of CD 34 was performed by QRT-PCR method.Results: Combination of 5% of UCB-PL with mentioned cytokines yielded a 12-fold cell proliferation in comparison with control group. A higher concentration of UCB-PL showed cytotoxic effects. CD 34 also increased significantly in group of 5% UCB-PL treated cells. In this particular group, a significant increase of colony forming units granulocytes-erythroidmacrophages-megakaryocyte (CFU-GMMM) and granulocyte-macrophage (CFU-GM) were detected.Conclusion: Our results indicate that 5% UCB -PL can be used as a supplement in MNC cultured cells and in cord blood hematopoietic stem cells for cell therapy purposes.

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APA: Copy

MOHAMMAD, MONIREH, EBRAHIMI, MARZIEH, DANESHMAND, FATEMEH, NOURI, MASOUMEH, & KHOSRAVANI, PARDIS. (2017). EX VIVO EXPANSION OF CORD BLOOD MONONUCLEAR CELLS BY UMBILICAL CORD BLOOD PLATELET LYSATE. DANESHVAR MEDICINE, 24(127 ), 49-61. SID. https://sid.ir/paper/30588/en

Vancouver: Copy

MOHAMMAD MONIREH, EBRAHIMI MARZIEH, DANESHMAND FATEMEH, NOURI MASOUMEH, KHOSRAVANI PARDIS. EX VIVO EXPANSION OF CORD BLOOD MONONUCLEAR CELLS BY UMBILICAL CORD BLOOD PLATELET LYSATE. DANESHVAR MEDICINE[Internet]. 2017;24(127 ):49-61. Available from: https://sid.ir/paper/30588/en

IEEE: Copy

MONIREH MOHAMMAD, MARZIEH EBRAHIMI, FATEMEH DANESHMAND, MASOUMEH NOURI, and PARDIS KHOSRAVANI, “EX VIVO EXPANSION OF CORD BLOOD MONONUCLEAR CELLS BY UMBILICAL CORD BLOOD PLATELET LYSATE,” DANESHVAR MEDICINE, vol. 24, no. 127 , pp. 49–61, 2017, [Online]. Available: https://sid.ir/paper/30588/en

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