The Viability of Human Testis-Derived Cells on Human Serum Albumin-Based Scaffold as An Artificial Male Germ Cell Niche

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Borzouie Zahra; HEKMATI MOGHADDAM SEYEDHOSSEIN; JEBALI ALI; AFLATOONIAN BEHROUZ

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Journal Paper

Paper Information

Journal: International Journal of Fertility & Sterility Year:2020 | Volume:14 | Issue:2 Page(s): 150-153

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Title

The Viability of Human Testis-Derived Cells on Human Serum Albumin-Based Scaffold as An Artificial Male Germ Cell Niche

Author(s)

Borzouie Zahra | HEKMATI MOGHADDAM SEYEDHOSSEIN | JEBALI ALI | AFLATOONIAN BEHROUZ | Issue Writer Certificate

Keywords

Azoospermia

Human Serum Albumin

Scaffold

Spermatogenesis

Testis

Abstract

Azoospermia is one of the challenging disorders affecting couples who are afflicted with infertility. Human Testisderived cells (hTCs) are suitable candidates for the initiation of in-vitro Spermatogenesis for these types of patients. The current study aimed to assess the proliferation of hTCs through the cell culture on the three-dimensional (3D) porous Scaffolds. Cells harvested from the testicular sperm extraction (TESE) samples of the azoospermic patients were cultured on the 3D porous Scaffolds containing Human Serum Albumin (HSA)/tri calcium phosphate nanoparticles (TCP NPs) for two weeks. The proliferation/viability of the cells was assessed using the MTT assay, along with H&E histological staining method. The MTT assay showed that hTCs could stay alive on this Scaffold with 50 and 66. 66% viability after 7 and 14 days, respectively. Such viability was not significantly different when compared with cells grown on monolayer flask culture (P>0. 05). Therefore, 3D HSA/TCP NPs Scaffolds could be used for the reconstitution of the artificial human somatic testicular niche for future applications in regenerative medicine for male infertility.

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APA: Copy

Borzouie, Zahra, HEKMATI MOGHADDAM, SEYEDHOSSEIN, & JEBALI, ALI. (2020). The Viability of Human Testis-Derived Cells on Human Serum Albumin-Based Scaffold as An Artificial Male Germ Cell Niche. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, 14(2), 150-153. SID. https://sid.ir/paper/306099/en

Vancouver: Copy

Borzouie Zahra, HEKMATI MOGHADDAM SEYEDHOSSEIN, JEBALI ALI. The Viability of Human Testis-Derived Cells on Human Serum Albumin-Based Scaffold as An Artificial Male Germ Cell Niche. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY[Internet]. 2020;14(2):150-153. Available from: https://sid.ir/paper/306099/en

IEEE: Copy

Zahra Borzouie, SEYEDHOSSEIN HEKMATI MOGHADDAM, and ALI JEBALI, “The Viability of Human Testis-Derived Cells on Human Serum Albumin-Based Scaffold as An Artificial Male Germ Cell Niche,” INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, vol. 14, no. 2, pp. 150–153, 2020, [Online]. Available: https://sid.ir/paper/306099/en

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