UBIQUITIN CONJUGATION OF HEPATITIS B VIRUS CORE ANTIGEN DNA VACCINE LEADS TO ENHANCED CELL-MEDIATED IMMUNE RESPONSE IN BALB/C MICE

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CHEN JIAN HUA; YU YONG SHENG; LIU HONG HONG; CHEN XIAO HUA; XI MIN; ZANG GUO QING; TANG ZHENG HAO

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Journal: Hepatitis Monthly Year:2011 | Volume:11 | Issue:8 (37) Page(s): 620-628

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Title

UBIQUITIN CONJUGATION OF HEPATITIS B VIRUS CORE ANTIGEN DNA VACCINE LEADS TO ENHANCED CELL-MEDIATED IMMUNE RESPONSE IN BALB/C MICE

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Keywords

DNA VACCINE

UBIQUITIN

HEPATITIS B CORE ANTIGEN

Abstract

Background: Nearly 350 million persons worldwide are chronically infected with hepatitis B virus (HBV). UBIQUITIN (Ub) is a highly conserved small regulatory protein, ubiquitous in eukaryotes, that usually serves as a signal for the target protein that is recognised and degraded in proteasomes. The Ub-mediated processing of antigens is rapid and efficient and stimulates cell-mediated immune responses. Accordingly, Ubmediated processing of antigens has been widely used in chronic-infection and cancer studies to improve immune response.Objectives: Many clinical trials have shown that DNA VACCINE potency needs to be greatly enhanced. Here, we report a new strategy for designing an HBV DNA VACCINE using the UBIQUITIN (Ub) sequence. The aim of this study was to investigate a novel DNA vaccination, based on the expression of HBV core antigen (HBcAg), fused to Ub to enhance DNA VACCINE potency.Materials and Methods: Mouse UBIQUITIN fused to the HBcAg gene and cloned into the eukaryotic vector pcDNA3.1 (-). BALB/c mice were immunized with recombinant pUb- HBcAg or pHBcAg DNA VACCINE. Lymphocyte proliferation assay, intracellular IFN-γ assay, CTL cytotoxicity assay, and antibody assay were performed to analyze the cellular and humoral immune responses to our DNA constructs.Results: HBcAg was expressed effectively in the COS-7 cells that were transiently transfected with pUb-HBcAg. Strong anti-HBc IgG responses were elicited in mice that were immunized with pUb-HBcAg. The endpoint titers of anti-HBc peaked at 1: 656100 on the 42nd day after the third immunization. pUb-HBcAg stimulated greater lymphocyte proliferation and induced higher levels of IL-2 and IFN-g and a greater percentage of HBcAg-specific CD8+T cells in mice than pHBcAg. In the CTL assay, the specific lysis rate reached 56.5% at an effector: target ratio of 50: 1 in mice that were immunized with pUb-HBcAg.Conclusions: pUb-HBcAg elicits specific anti-HBc responses and induces HBc-specific CTL responses in immunized BALB/c mice. Our results imply that Ub can be used as a molecular adjuvant that enhances the potency of DNA VACCINEs.

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APA: Copy

CHEN, JIAN HUA, YU, YONG SHENG, LIU, HONG HONG, CHEN, XIAO HUA, XI, MIN, ZANG, GUO QING, & TANG, ZHENG HAO. (2011). UBIQUITIN CONJUGATION OF HEPATITIS B VIRUS CORE ANTIGEN DNA VACCINE LEADS TO ENHANCED CELL-MEDIATED IMMUNE RESPONSE IN BALB/C MICE. HEPATITIS MONTHLY, 11(8 (37)), 620-628. SID. https://sid.ir/paper/306437/en

Vancouver: Copy

CHEN JIAN HUA, YU YONG SHENG, LIU HONG HONG, CHEN XIAO HUA, XI MIN, ZANG GUO QING, TANG ZHENG HAO. UBIQUITIN CONJUGATION OF HEPATITIS B VIRUS CORE ANTIGEN DNA VACCINE LEADS TO ENHANCED CELL-MEDIATED IMMUNE RESPONSE IN BALB/C MICE. HEPATITIS MONTHLY[Internet]. 2011;11(8 (37)):620-628. Available from: https://sid.ir/paper/306437/en

IEEE: Copy

JIAN HUA CHEN, YONG SHENG YU, HONG HONG LIU, XIAO HUA CHEN, MIN XI, GUO QING ZANG, and ZHENG HAO TANG, “UBIQUITIN CONJUGATION OF HEPATITIS B VIRUS CORE ANTIGEN DNA VACCINE LEADS TO ENHANCED CELL-MEDIATED IMMUNE RESPONSE IN BALB/C MICE,” HEPATITIS MONTHLY, vol. 11, no. 8 (37), pp. 620–628, 2011, [Online]. Available: https://sid.ir/paper/306437/en

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