HEV-ORF3 ENCODING PHOSPHOPROTEIN INTERACTS WITH HEPSIN

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WANG CHUNYAN; GUO LIANG; YU DAYI; HUA XIUGUO; YANG ZHIBIAO; YUAN CONGLI; CUI LI

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Journal Paper

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Journal: Hepatitis Monthly Year:2014 | Volume:14 | Issue:1 Page(s): 1-4

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Title

HEV-ORF3 ENCODING PHOSPHOPROTEIN INTERACTS WITH HEPSIN

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Keywords

ORF3 PROTEIN

HEPATITIS E VIRUS

HEPSIN

TWO-HYBRID SYSTEM TECHNIQUES

IMMUNOPRECIPITATION

Abstract

Background: HEPATITIS E VIRUS (HEV) is a major causative agent of acute clinical hepatitis in adults through much of Asia, the Middle East and Africa. Open reading frame 3 (ORF3) encodes around 120 amino acids of phosphorylation protein that associates with the cytoskeleton, while its precise biological function is still unknown.Objectives: In order to understand the function of ORF3 PROTEIN (pORF3) in depth, HEV ORF3 interacting proteins were screened in human hepatocytes cDNA library using TWO-HYBRID SYSTEM TECHNIQUES and further verification of the interactions were carried out through co-immunoprecipitation (Co-IP).Materials and Methods: The Cyto-Trap two-hybrid system technology, a classical method for analyzing protein interactions, was used to screen the pORF3 interacting proteins from human hepatocytes cDNA library.Results: Through the Cyto-Trap two-hybrid system, eight proteins interacting with pORF3 were winnowed. The Co-IP results confirmed that HEPSIN which is reported to function as the inhibitor of several tumors reacted with pORF3.Conclusions: Out of eight screened proteins interacting with pORF3, HEPSIN was confirmed to have specific interactions with pORF3.

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APA: Copy

WANG, CHUNYAN, GUO, LIANG, YU, DAYI, HUA, XIUGUO, YANG, ZHIBIAO, YUAN, CONGLI, & CUI, LI. (2014). HEV-ORF3 ENCODING PHOSPHOPROTEIN INTERACTS WITH HEPSIN. HEPATITIS MONTHLY, 14(1), 1-4. SID. https://sid.ir/paper/306592/en

Vancouver: Copy

WANG CHUNYAN, GUO LIANG, YU DAYI, HUA XIUGUO, YANG ZHIBIAO, YUAN CONGLI, CUI LI. HEV-ORF3 ENCODING PHOSPHOPROTEIN INTERACTS WITH HEPSIN. HEPATITIS MONTHLY[Internet]. 2014;14(1):1-4. Available from: https://sid.ir/paper/306592/en

IEEE: Copy

CHUNYAN WANG, LIANG GUO, DAYI YU, XIUGUO HUA, ZHIBIAO YANG, CONGLI YUAN, and LI CUI, “HEV-ORF3 ENCODING PHOSPHOPROTEIN INTERACTS WITH HEPSIN,” HEPATITIS MONTHLY, vol. 14, no. 1, pp. 1–4, 2014, [Online]. Available: https://sid.ir/paper/306592/en

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