IMMUNOGENICITY OF MULTI-EPITOPE DNA AND PEPTIDE VACCINE CANDIDATES BASED ON CORE, E2, NS3 AND NS5B HCV EPITOPES IN BALB/C MICE

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LEILA PISHRAFT SABET; TAHERI TAHEREH; MEMARNEJADIAN ARASH; MOKHTARI AZAD TALAT; ASGARI FATEMEH; RAHIMNIA RAMIN; ALAVIAN SEYED MOAYED; RAFATI SIMA; SAMIMI RAD KATAYOUN

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Journal: Hepatitis Monthly Year:2014 | Volume:14 | Issue:10 Page(s): 0-0

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Title

IMMUNOGENICITY OF MULTI-EPITOPE DNA AND PEPTIDE VACCINE CANDIDATES BASED ON CORE, E2, NS3 AND NS5B HCV EPITOPES IN BALB/C MICE

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Keywords

VACCINE

EPITOPE

ELECTROPORATION

PRIME-BOOST

Abstract

Background: Hypervariability of HCV proteins is an important obstacle to design an efficient VACCINE for HCV infection. Multi-epitope VACCINEs containing conserved EPITOPEs of the virus could be a promising approach for protection against HCV.Objectives: Cellular and humoral immune responses against multi-epitope DNA and peptide VACCINEs were evaluated in BALB/c mice.Materials and Methods: In this experimental study, multi-epitope DNA- and peptide-based VACCINEs for HCV infection harboring immunodominant CD8+T cell EPITOPEs (HLA-A2 and H2-Dd) from Core (132-142), NS3 (1073-1081) and NS5B (2727-2735), a Th CD4+epitope from NS3 (1248-1262) and a B-cell EPITOPE from E2 (412-426) were designed. Multi-epitope DNA and peptide VACCINEs were tested in two regimens as heterologous DNA/peptide (group 1) and homologous peptide/peptide (group 2) prime/boost VACCINE in BALB/c mice model. ELECTROPORATION was used for delivery of the DNA VACCINE. Peptide VACCINE was formulated with Montanide ISA 720 (M720) as adjuvant. Cytokine assay and antibody detection were performed to analyze the immune responses.Results: Mice immunized with multi-epitope peptide formulated with M720 developed higher HCV-specific levels of total IgG, IgG1 and IgG2a than those immunized with multi-epitope DNA VACCINE. IFN-γ levels in group 2 were significantly higher than group 1 (i.e.3 weeks after the last immunization, 37.61±2.39 vs.14.43±0.43, P<0.05). Moreover, group 2 had a higher IFN-Y/IL-4 ratio compared to group 1, suggesting a shift toward Th1 response. In addition, in the present study, induced immune responses were long lasting and stable after 9 weeks of the last immunization.Conclusions: Evaluation of multi-epitope DNA and peptide-VACCINEs confirmed their specific immunogenicity in BALB/c mice. However, lower Th1 immune responses in mice immunized with DNA VACCINE suggests further investigations to improve the immunogenicity of the multi-epitope DNA VACCINE through immune enhancers.

Cites

EXPRESSION OF NS3/NS4A PROTEINS OF HEPATITIS C VIRUS IN HUH7 CELLS FOLLOWING ENGINEERING ITS EUKARYOTIC EXPRESSION VECTOR

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APA: Copy

LEILA, PISHRAFT SABET, TAHERI, TAHEREH, MEMARNEJADIAN, ARASH, MOKHTARI AZAD, TALAT, ASGARI, FATEMEH, RAHIMNIA, RAMIN, ALAVIAN, SEYED MOAYED, RAFATI, SIMA, & SAMIMI RAD, KATAYOUN. (2014). IMMUNOGENICITY OF MULTI-EPITOPE DNA AND PEPTIDE VACCINE CANDIDATES BASED ON CORE, E2, NS3 AND NS5B HCV EPITOPES IN BALB/C MICE. HEPATITIS MONTHLY, 14(10), 0-0. SID. https://sid.ir/paper/306609/en

Vancouver: Copy

LEILA PISHRAFT SABET, TAHERI TAHEREH, MEMARNEJADIAN ARASH, MOKHTARI AZAD TALAT, ASGARI FATEMEH, RAHIMNIA RAMIN, ALAVIAN SEYED MOAYED, RAFATI SIMA, SAMIMI RAD KATAYOUN. IMMUNOGENICITY OF MULTI-EPITOPE DNA AND PEPTIDE VACCINE CANDIDATES BASED ON CORE, E2, NS3 AND NS5B HCV EPITOPES IN BALB/C MICE. HEPATITIS MONTHLY[Internet]. 2014;14(10):0-0. Available from: https://sid.ir/paper/306609/en

IEEE: Copy

PISHRAFT SABET LEILA, TAHEREH TAHERI, ARASH MEMARNEJADIAN, TALAT MOKHTARI AZAD, FATEMEH ASGARI, RAMIN RAHIMNIA, SEYED MOAYED ALAVIAN, SIMA RAFATI, and KATAYOUN SAMIMI RAD, “IMMUNOGENICITY OF MULTI-EPITOPE DNA AND PEPTIDE VACCINE CANDIDATES BASED ON CORE, E2, NS3 AND NS5B HCV EPITOPES IN BALB/C MICE,” HEPATITIS MONTHLY, vol. 14, no. 10, pp. 0–0, 2014, [Online]. Available: https://sid.ir/paper/306609/en

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