HAEMATOLOGIC AND MITOGENIC PROPERTIES OF PHASEOLUS COCCINEUS AGGLUTININ

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TAVASOLI N.; MIRSHAFIEI A.; SOLTAN DALAL MOHAMMAD MAHDI; NASEH M.

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Journal: Daneshvar Medicine: Basic and Clinical Research Journal Year:2003 | Volume:11 | Issue:47 Page(s): 15-22

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Title

HAEMATOLOGIC AND MITOGENIC PROPERTIES OF PHASEOLUS COCCINEUS AGGLUTININ

Author(s)

TAVASOLI N. | MIRSHAFIEI A. | SOLTAN DALAL MOHAMMAD MAHDI | NASEH M. | Issue Writer Certificate

Keywords

PHASEOLUS COCCINEUS-LECTIN-MITOGENIC-HAEMAGGLUTINATIONQ4

Abstract

The aims of the present study were isolation of lectin from phaseolus coccineus seeds, determination of its minimal concentration in haemagglutination process and evaluation of its mitogenic efficacy in stimulation of cell proliferation.The lectin was extracted from the seeds by the reported method of Nagata et al. The amount of isolated protein was 130mg/ml which was measured by the method of Biuret.The minimal concentration of the phaseolus coccineus lectin which was able to cause visible agglutination of 5% human red blood cell suspension was about 0.125mg/ml. This Agglutinin could cause agglutination of all human RBC irrespective of their ABO blood group system.The mitogenic activity of this lectin was measured by lymphocyte transformation test (L.T.T.). The result showed that the isolated lecti was strongly mitogenic for human lymphocyte. The mitogenic activity of 100mg/ml of phaseolus coccineus lectin was equal to the mitogenic activity of the same concentration of PHA (phytohaemagglutinin) which is used routinely in immunologic tests.

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APA: Copy

TAVASOLI, N., MIRSHAFIEI, A., SOLTAN DALAL, MOHAMMAD MAHDI, & NASEH, M.. (2003). HAEMATOLOGIC AND MITOGENIC PROPERTIES OF PHASEOLUS COCCINEUS AGGLUTININ. DANESHVAR MEDICINE, 11(47), 15-22. SID. https://sid.ir/paper/30663/en

Vancouver: Copy

TAVASOLI N., MIRSHAFIEI A., SOLTAN DALAL MOHAMMAD MAHDI, NASEH M.. HAEMATOLOGIC AND MITOGENIC PROPERTIES OF PHASEOLUS COCCINEUS AGGLUTININ. DANESHVAR MEDICINE[Internet]. 2003;11(47):15-22. Available from: https://sid.ir/paper/30663/en

IEEE: Copy

N. TAVASOLI, A. MIRSHAFIEI, MOHAMMAD MAHDI SOLTAN DALAL, and M. NASEH, “HAEMATOLOGIC AND MITOGENIC PROPERTIES OF PHASEOLUS COCCINEUS AGGLUTININ,” DANESHVAR MEDICINE, vol. 11, no. 47, pp. 15–22, 2003, [Online]. Available: https://sid.ir/paper/30663/en

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