HEPATOCYTE ISOLATION FROM UNUSED/REJECTED LIVERS FOR TRANSPLANTATION: INITIAL STEP TOWARD HEPATOCYTE TRANSPLANTATION, THE FIRST EXPERIENCE FROM IRAN

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HOSSEIN AGHDAIE MAHDOKHT; GERAMIZADEH BITA; AZARPIRA NEGAR; ESFANDIARI ELAHEH; DARAI MASOUME; RAHSAZ MARJAN; NIKEGHBALIAN SAMAN; MALEKHOSSEINI SEYED ALI

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Journal: Hepatitis Monthly Year:2013 | Volume:13 | Issue:8 Page(s): 1-5

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Title

HEPATOCYTE ISOLATION FROM UNUSED/REJECTED LIVERS FOR TRANSPLANTATION: INITIAL STEP TOWARD HEPATOCYTE TRANSPLANTATION, THE FIRST EXPERIENCE FROM IRAN

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Keywords

HEPATOCYTE

LIVER

TRANSPLANTATION

Abstract

Background: HEPATOCYTE TRANSPLANTATION is being used in patients with LIVER-based metabolic disorders and acute LIVER failure. HEPATOCYTEs can be isolated from unused/rejected LIVERs under sterile conditions.Objectives: The quality of the HEPATOCYTEs is very important and the main and initial step in HEPATOCYTE TRANSPLANTATION is HEPATOCYTE isolation. In this study we tried to set up the methods of HEPATOCYTE isolation in order to use the high quality cells in acute LIVER failure or congenital metabolic disorders.Materials and Methods: In this study, during a year, HEPATOCYTEs were isolated from 7 unused/rejected LIVERs among more than 300 harvested LIVERs in Shiraz University of Medical Sciences. The two step collagenase perfusion method was used under GMP (Good manufacturing practice) for HEPATOCYTE isolation.Result: Highly quality HEPATOCYTEs with high viability and low contamination were isolated. The mean viability was 71.8%±21.7. In the first 4 cases microbial contamination by Staphylococci, Diphtheroid and Klebsiella was detected, however the last 3 cases were free of any microorganisms. After 5 weeks of cryopreservation in -140oC, the cell viability was still acceptable.Conclusion: HEPATOCYTE isolation can be performed as the main and initial step for cell TRANSPLANTATION from unused/rejected LIVER. It is the first experience in Iran.

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APA: Copy

HOSSEIN AGHDAIE, MAHDOKHT, GERAMIZADEH, BITA, AZARPIRA, NEGAR, ESFANDIARI, ELAHEH, DARAI, MASOUME, RAHSAZ, MARJAN, NIKEGHBALIAN, SAMAN, & MALEKHOSSEINI, SEYED ALI. (2013). HEPATOCYTE ISOLATION FROM UNUSED/REJECTED LIVERS FOR TRANSPLANTATION: INITIAL STEP TOWARD HEPATOCYTE TRANSPLANTATION, THE FIRST EXPERIENCE FROM IRAN. HEPATITIS MONTHLY, 13(8), 1-5. SID. https://sid.ir/paper/306675/en

Vancouver: Copy

HOSSEIN AGHDAIE MAHDOKHT, GERAMIZADEH BITA, AZARPIRA NEGAR, ESFANDIARI ELAHEH, DARAI MASOUME, RAHSAZ MARJAN, NIKEGHBALIAN SAMAN, MALEKHOSSEINI SEYED ALI. HEPATOCYTE ISOLATION FROM UNUSED/REJECTED LIVERS FOR TRANSPLANTATION: INITIAL STEP TOWARD HEPATOCYTE TRANSPLANTATION, THE FIRST EXPERIENCE FROM IRAN. HEPATITIS MONTHLY[Internet]. 2013;13(8):1-5. Available from: https://sid.ir/paper/306675/en

IEEE: Copy

MAHDOKHT HOSSEIN AGHDAIE, BITA GERAMIZADEH, NEGAR AZARPIRA, ELAHEH ESFANDIARI, MASOUME DARAI, MARJAN RAHSAZ, SAMAN NIKEGHBALIAN, and SEYED ALI MALEKHOSSEINI, “HEPATOCYTE ISOLATION FROM UNUSED/REJECTED LIVERS FOR TRANSPLANTATION: INITIAL STEP TOWARD HEPATOCYTE TRANSPLANTATION, THE FIRST EXPERIENCE FROM IRAN,” HEPATITIS MONTHLY, vol. 13, no. 8, pp. 1–5, 2013, [Online]. Available: https://sid.ir/paper/306675/en

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