IN VITRO ANALYSIS OF CSA-INDUCED HEPATOTOXICITY IN HEPG2 CELL LINE: OXIDATIVE STRESS AND α2 AND β1 INTEGRIN SUBUNITS EXPRESSION

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MOSTAFAVI POUR ZOHREH; KHADEMI FATEMEH; ZAL FATEMEH; SARDARIAN AHMADREZA; AMINI FATEMEH

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Journal: Hepatitis Monthly Year:2013 | Volume:13 | Issue:8 Page(s): 1-8

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Title

IN VITRO ANALYSIS OF CSA-INDUCED HEPATOTOXICITY IN HEPG2 CELL LINE: OXIDATIVE STRESS AND α2 AND β1 INTEGRIN SUBUNITS EXPRESSION

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Keywords

CYCLOSPORINE

ANTIOXIDANT

ENZYMES

INTEGRIN ALPHA2BETA1

Abstract

Background: CYCLOSPORINE A (CsA)-induced hepatotoxicity could be due to a reduction in a2b1 integrin expression that may either be from the direct effect of CsA itself or from reactive oxygen species (ROS) overproduction.Objectives: In this study we aimed to identify the cellular mechanisms underlying CsA-induced hepatic injury by investigating the activation patterns of the ANTIOXIDANT ENZYMES, using HepG2 as an in vitro model.Materials and Methods: HepG2 cells were cultured with different concentrations of CsA (0, 0.1, 1, 10 mg/ml) for 72 h. Effect of CsA on, 1) cellular integrity, 2) glutathione reductase (GR) and glutathione peroxidase (GPx) activity, 3) cellular levels of glutathione (GSH), 4) intracellular ROS, 5) ALT and AST activities, 6) urea production and 7) a2b1 integrin expression were assayed.Results: CsA treatment demonstrated a dose dependent increase in intracellular levels of ROS, GPx activity and decrease in GSH levels (P<0.05). GR activity was mildly attenuated in 1 and 10 mg/ml concentrations of CsA. Alanine aminotranferase (ALT) and aspartate aminotransferase (AST) levels increased in CsA treated cells, while urea synthesis was significantly decreased following treatment with higher concentrations of CsA (P<0.05). Significant down-regulation of β1integrin expression was observed in 1 and 10 mg/ml CsA treated cells while α2 integrin mRNA was significantly down-regulated in all CsA treated cells.Conclusions: The observed reduction of a2b1 integrin expression following CsA treatment could be proposed as a possible pathway of CsA-induced hepatotoxicity. Further studies are required to elucidate whether this attenuated expression is due to the direct effect of CsA or caused by overproduction of ROS.

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APA: Copy

MOSTAFAVI POUR, ZOHREH, KHADEMI, FATEMEH, ZAL, FATEMEH, SARDARIAN, AHMADREZA, & AMINI, FATEMEH. (2013). IN VITRO ANALYSIS OF CSA-INDUCED HEPATOTOXICITY IN HEPG2 CELL LINE: OXIDATIVE STRESS AND α2 AND β1 INTEGRIN SUBUNITS EXPRESSION. HEPATITIS MONTHLY, 13(8), 1-8. SID. https://sid.ir/paper/306686/en

Vancouver: Copy

MOSTAFAVI POUR ZOHREH, KHADEMI FATEMEH, ZAL FATEMEH, SARDARIAN AHMADREZA, AMINI FATEMEH. IN VITRO ANALYSIS OF CSA-INDUCED HEPATOTOXICITY IN HEPG2 CELL LINE: OXIDATIVE STRESS AND α2 AND β1 INTEGRIN SUBUNITS EXPRESSION. HEPATITIS MONTHLY[Internet]. 2013;13(8):1-8. Available from: https://sid.ir/paper/306686/en

IEEE: Copy

ZOHREH MOSTAFAVI POUR, FATEMEH KHADEMI, FATEMEH ZAL, AHMADREZA SARDARIAN, and FATEMEH AMINI, “IN VITRO ANALYSIS OF CSA-INDUCED HEPATOTOXICITY IN HEPG2 CELL LINE: OXIDATIVE STRESS AND α2 AND β1 INTEGRIN SUBUNITS EXPRESSION,” HEPATITIS MONTHLY, vol. 13, no. 8, pp. 1–8, 2013, [Online]. Available: https://sid.ir/paper/306686/en

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