DEVELOPMENT OF MAGNETIC CAPTURE HYBRIDIZATION AND QUANTITATIVE POLYMERASE CHAIN REACTION FOR HEPATITIS B VIRUS COVALENTLY CLOSED CIRCULAR DNA

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GUO YONGCAN; SHENG SHANGCHUN; NIE BIN; ZHIGUANG TU

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Journal Paper

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Journal: Hepatitis Monthly Year:2015 | Volume:15 | Issue:1 Page(s): 0-0

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Title

DEVELOPMENT OF MAGNETIC CAPTURE HYBRIDIZATION AND QUANTITATIVE POLYMERASE CHAIN REACTION FOR HEPATITIS B VIRUS COVALENTLY CLOSED CIRCULAR DNA

Author(s)

GUO YONGCAN | SHENG SHANGCHUN | NIE BIN | ZHIGUANG TU | Issue Writer Certificate

Keywords

HEPATITIS B VIRUS

MAGNETIC

PCR TECHNOLOGY

Abstract

Background: HEPATITIS B VIRUS (HBV) covalently closed circular DNA (cccDNA) served as a vital role in the life cycle of the virus and persistent infection. However, specific and quantitative methods for cccDNA detection have not been available. Objectives: Our aim was to develop and primarily evaluate a quantitative method for HBV cccDNA based on MAGNETIC capture hybridization and quantitative PCR TECHNOLOGY.Materials and Methods: The functionalized-nanoparticles specifically to capture HBV cccDNA, located on both sides of relaxed circle DNA (rcDNA) gap, were designed. Then, MAGNETIC capture hybridization and quantitative PCR (MCH-qPCR) assay were developed and its performance was primarily evaluated with cccDNA standards and serum samples of patients with chronic hepatitis B. Results: Specific nanoparticles of cccDNA capture were prepared and a MAGNETIC capture hybridization and quantitative assay method for cccDNA was developed successfully. The limit of detection was 90 IU/mL, and a good linear relationship in the range of 102-106 IU/mL was revealed (r2=0.994) with the MCH-qPCR. Compared with directly real-time PCR, a high content of HBV DNA did not affect the detection of cccDNA for the MCH-qPCR method, and there was no cross-reactivity between cccDNA and rcDNA.Conclusions: The novel MCH-qPCR method has good sensitivity and specificity. It could meet the requirement of clinical routine detection.

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APA: Copy

GUO, YONGCAN, SHENG, SHANGCHUN, NIE, BIN, & ZHIGUANG, TU. (2015). DEVELOPMENT OF MAGNETIC CAPTURE HYBRIDIZATION AND QUANTITATIVE POLYMERASE CHAIN REACTION FOR HEPATITIS B VIRUS COVALENTLY CLOSED CIRCULAR DNA. HEPATITIS MONTHLY, 15(1), 0-0. SID. https://sid.ir/paper/306884/en

Vancouver: Copy

GUO YONGCAN, SHENG SHANGCHUN, NIE BIN, ZHIGUANG TU. DEVELOPMENT OF MAGNETIC CAPTURE HYBRIDIZATION AND QUANTITATIVE POLYMERASE CHAIN REACTION FOR HEPATITIS B VIRUS COVALENTLY CLOSED CIRCULAR DNA. HEPATITIS MONTHLY[Internet]. 2015;15(1):0-0. Available from: https://sid.ir/paper/306884/en

IEEE: Copy

YONGCAN GUO, SHANGCHUN SHENG, BIN NIE, and TU ZHIGUANG, “DEVELOPMENT OF MAGNETIC CAPTURE HYBRIDIZATION AND QUANTITATIVE POLYMERASE CHAIN REACTION FOR HEPATITIS B VIRUS COVALENTLY CLOSED CIRCULAR DNA,” HEPATITIS MONTHLY, vol. 15, no. 1, pp. 0–0, 2015, [Online]. Available: https://sid.ir/paper/306884/en

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