A NOVEL HYDRODYNAMIC INJECTION MOUSE MODEL OF HBV GENOTYPE C FOR THE STUDY OF HBV BIOLOGY AND THE ANTI-VIRAL ACTIVITY OF LAMIVUDINE

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LI XIUMEI; LIU GUANGZE; CHEN MEIJUAN; YANG YANG; XIE YONG; KONG XIANGPING

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Journal: Hepatitis Monthly Year:2016 | Volume:16 | Issue:2 Page(s): 0-0

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Title

A NOVEL HYDRODYNAMIC INJECTION MOUSE MODEL OF HBV GENOTYPE C FOR THE STUDY OF HBV BIOLOGY AND THE ANTI-VIRAL ACTIVITY OF LAMIVUDINE

Author(s)

Keywords

HEPATITIS B VIRUS

INTRAVENOUS

INJECTIONS

MODELS

ANIMAL

LAMIVUDINE

Abstract

Background: Absence of an immunocompetent mouse model of persistent HEPATITIS B VIRUS (HBV) infection has hindered the research of HBV infection and the development of antiviral medications.Objectives: In the present study, we aimed to develop a novel HBV genotype C mouse model by hydrodynamic injection (HI) and then used it to evaluate the antiviral activity of LAMIVUDINE.Materials and Methods: A quantity of 15 mg of HBV plasmid [pcDNA3.1 (+) -HBV1.3C], adeno-associated virus-HBV1.3C (pAAV-HBV1.3C) or pAAV-HBV1.2A) were injected into male C57BL/6 mice, by HI, accounting for a total of 13 mice per group. Then, LAMIVUDINE was administered to mice with sustained HBV viremia, for 4 weeks. Real-time polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry methods were used to detect HBsAg, HBeAg, HBsAb, HBcAg and HBV DNA, in serum or liver of the mice, at indicated time points.Results: In 60% of the mice injected with pcDNA3.1 (+) -HBV1.3C, HBsAg, HBeAg, HBcAg and HBV DNA persisted for>20 weeks in liver, post-injection, with no HBsAb appearance. Meanwhile, no significant inflammation was observed in these mice. Compared with pAAV-HBV1.2A and pAAV-HBV1.3C, pcDNA3.1 (+) -HBV1.3C administration led to higher and longer HBV viremia. Furthermore, serum HBV DNA was significantly reduced by LAMIVUDINE, after 4 weeks administration, and returned to the original level, after ceasing administration for 1 week, in the mice.Conclusions: In conclusion, our observations indicated that pcDNA3.1 (+) -HBV1.3C was superior to AAV/HBV plasmid for establishment of persistent HBV infection by HI, in vivo, and this mouse model could be useful for studies of hepatitis virology and for the development of innovatory treatments for HBV infections.

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References

RELATIONSHIP BETWEEN HBV GENOTYPES B, C AND FOLLICULAR HELPER T CELLS IN PATIENTS WITH CHRONIC HEPATITIS B AND ITS SIGNIFICANCE

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APA: Copy

LI, XIUMEI, LIU, GUANGZE, CHEN, MEIJUAN, YANG, YANG, XIE, YONG, & KONG, XIANGPING. (2016). A NOVEL HYDRODYNAMIC INJECTION MOUSE MODEL OF HBV GENOTYPE C FOR THE STUDY OF HBV BIOLOGY AND THE ANTI-VIRAL ACTIVITY OF LAMIVUDINE. HEPATITIS MONTHLY, 16(2), 0-0. SID. https://sid.ir/paper/306974/en

Vancouver: Copy

LI XIUMEI, LIU GUANGZE, CHEN MEIJUAN, YANG YANG, XIE YONG, KONG XIANGPING. A NOVEL HYDRODYNAMIC INJECTION MOUSE MODEL OF HBV GENOTYPE C FOR THE STUDY OF HBV BIOLOGY AND THE ANTI-VIRAL ACTIVITY OF LAMIVUDINE. HEPATITIS MONTHLY[Internet]. 2016;16(2):0-0. Available from: https://sid.ir/paper/306974/en

IEEE: Copy

XIUMEI LI, GUANGZE LIU, MEIJUAN CHEN, YANG YANG, YONG XIE, and XIANGPING KONG, “A NOVEL HYDRODYNAMIC INJECTION MOUSE MODEL OF HBV GENOTYPE C FOR THE STUDY OF HBV BIOLOGY AND THE ANTI-VIRAL ACTIVITY OF LAMIVUDINE,” HEPATITIS MONTHLY, vol. 16, no. 2, pp. 0–0, 2016, [Online]. Available: https://sid.ir/paper/306974/en

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