ANALYSIS OF IMMUNOGLOBULIN HEAVY CHAIN GENE REARRANGEMENT USING PCR FOR EVALUATION OF CLONALITY & MRD (PRELIMINARY) IN CHILDREN WITH ACUTE LYMPHOBLASTIC LEUKEMIA

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POUPAK B.; POURFATH ELAH A.A.; YAHYAVI S.H.; NAJMABADI H.; MORTAZAVI YOUSEF; VOSOUGH PARVANEH; IZADYAR MINA; SHAHGHOLI E.; BAHOUSH GH.R.; HAGHNEZHAD DOUSHANLOU F.

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Journal: DANESHVAR MEDICINE Year:2005 | Volume:12 | Issue:57 Page(s): 21-26

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Title

ANALYSIS OF IMMUNOGLOBULIN HEAVY CHAIN GENE REARRANGEMENT USING PCR FOR EVALUATION OF CLONALITY & MRD (PRELIMINARY) IN CHILDREN WITH ACUTE LYMPHOBLASTIC LEUKEMIA

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Keywords

ACUTE LYMPHOBLASTIC LEUKEMIAQ4

CLONALITYQ4

MINIMAL RESIDUAL DISEASE (MRD)Q4

POLYMERASE CHAIN REACTIONQ4

Abstract

Introduction: Rearrangement of V, 0 & J segments of immunoglobulin heavy chain's gene with inserted or deleted nucleotides between rearranged segments make unique hyper variable regions (COR - 3).These regions can be used for evaluation of CLONALITY & minimal residual disease using PCR. Method: 71 children with diagnosis of B - precursor ACUTE LYMPHOBLASTIC LEUKEMIA who were admitted in Ali Asghar & Children Medical Center Hospitals were studied. After DNA extraction of mononuclear cells of bone marrow aspirates, PCR was done using consensus primers for amplification of COR - 3 region. PCR products were analyzed after heteroduplex analysis using polyacrylamide gel electrophoresis & silver staining. Results: Eighty two percent of patients showed clonal rearrangement. Sixty nine, twenty - four and seven percent of patients showed monoclonal, biclonal & oligoclonal patterns, respectively. Conclusion: As a result of acceptable sensitivity assay, evaluation of MRD is possible.

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APA: Copy

POUPAK, B., POURFATH ELAH, A.A., YAHYAVI, S.H., NAJMABADI, H., MORTAZAVI, YOUSEF, VOSOUGH, PARVANEH, IZADYAR, MINA, SHAHGHOLI, E., BAHOUSH, GH.R., & HAGHNEZHAD DOUSHANLOU, F.. (2005). ANALYSIS OF IMMUNOGLOBULIN HEAVY CHAIN GENE REARRANGEMENT USING PCR FOR EVALUATION OF CLONALITY & MRD (PRELIMINARY) IN CHILDREN WITH ACUTE LYMPHOBLASTIC LEUKEMIA. DANESHVAR MEDICINE, 12(57), 21-26. SID. https://sid.ir/paper/30735/en

Vancouver: Copy

POUPAK B., POURFATH ELAH A.A., YAHYAVI S.H., NAJMABADI H., MORTAZAVI YOUSEF, VOSOUGH PARVANEH, IZADYAR MINA, SHAHGHOLI E., BAHOUSH GH.R., HAGHNEZHAD DOUSHANLOU F.. ANALYSIS OF IMMUNOGLOBULIN HEAVY CHAIN GENE REARRANGEMENT USING PCR FOR EVALUATION OF CLONALITY & MRD (PRELIMINARY) IN CHILDREN WITH ACUTE LYMPHOBLASTIC LEUKEMIA. DANESHVAR MEDICINE[Internet]. 2005;12(57):21-26. Available from: https://sid.ir/paper/30735/en

IEEE: Copy

B. POUPAK, A.A. POURFATH ELAH, S.H. YAHYAVI, H. NAJMABADI, YOUSEF MORTAZAVI, PARVANEH VOSOUGH, MINA IZADYAR, E. SHAHGHOLI, GH.R. BAHOUSH, and F. HAGHNEZHAD DOUSHANLOU, “ANALYSIS OF IMMUNOGLOBULIN HEAVY CHAIN GENE REARRANGEMENT USING PCR FOR EVALUATION OF CLONALITY & MRD (PRELIMINARY) IN CHILDREN WITH ACUTE LYMPHOBLASTIC LEUKEMIA,” DANESHVAR MEDICINE, vol. 12, no. 57, pp. 21–26, 2005, [Online]. Available: https://sid.ir/paper/30735/en

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