THE EFFECT OF HUMAN CUMULUS CELL- CONDITIONED MEDIUM ON MOUSE EMBRYO DEVELOPMENT IN VITRO

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

KARIMPOUR A.; NEZHAD MOGHADAM A.A.; MOSAVI DARKA S.S.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Daneshvar Medicine: Basic and Clinical Research Journal Year:2006 | Volume:13 | Issue:63 Page(s): 75-80

Download Full-Text

Persian Verion

View:

934

Download:

Cites:

Information Journal Paper

Title

THE EFFECT OF HUMAN CUMULUS CELL- CONDITIONED MEDIUM ON MOUSE EMBRYO DEVELOPMENT IN VITRO

Author(s)

KARIMPOUR A. | NEZHAD MOGHADAM A.A. | MOSAVI DARKA S.S. | Issue Writer Certificate

Keywords

CUMULUS CELLQ3

CO-CULTUREQ3

CONDITIONED MEDIUMQ3

MOUSE EMBRYOQ3

Abstract

Introduction: Although numerous investigations have demonstrated the beneficial effects of CO-CULTURE system of different somatic cells on in vitro development of embryos, the effects of conditioned-media of CO-CULTURE cells have not been well documented yet. The objective of this study was to investigate the effects of human CUMULUS CELLs CO-CULTURE system and the CONDITIONED MEDIUM on the developmental rate of MOUSE EMBRYOs in vitro.Materials and Methods: Two sets of experiments were undertaken; in the first one 317 mouse one-cell embryos were cultured in human CUMULUS CELL CO-CULTURE system (GC), Ham's F10 medium conditioned with CUMULUS CELLs (CM) and non-conditioned Ham's F10 (HF), for 120 hours. In the second experiment, 391 late two-cell embryos were cultured in the 3 aforementioned culture treatments for 72 hours. Embryos were obtained from NMRI mice after superovulation using PMSG and hCG. Human CUMULUS CELLs were collected from patients undergoing an IVF program during oocyte pickup, 36 hours after hCG administration. CUMULUS CELLs in their FF were Pelleted by centrifugation and then were separated from red blood cells using a percoll gradient. Computation of X2 for different stages was done to compare their means.Results: 11, 24 and 14% of one-cell embryos passed two-cell block and continued growing to 4-cell in HF, GC and CM, respectively. This index in GC was significantly different from the two other treatments (p<0.05). Also, significantly more embryos reached blastocyst stage in GC compared to the two other treatments (p<0.05) and in CM compared to HF (p<0.05). In the second set of experiments the proportion of blastocyst stage was significantly higher in CM than the proportion in HF (p<0.05) and lower than that in GC (p<0.05).Conclusion: Based on the results, it can be concluded that although human CUMULUS CELL-conditioned medium has non-species specific beneficial effects on MOUSE EMBRYO development, it is not as effective as CO-CULTURE of these cells.

Cites

No record.

References

No record.

Cite

APA: Copy

KARIMPOUR, A., NEZHAD MOGHADAM, A.A., & MOSAVI DARKA, S.S.. (2006). THE EFFECT OF HUMAN CUMULUS CELL- CONDITIONED MEDIUM ON MOUSE EMBRYO DEVELOPMENT IN VITRO. DANESHVAR MEDICINE, 13(63), 75-80. SID. https://sid.ir/paper/30885/en

Vancouver: Copy

KARIMPOUR A., NEZHAD MOGHADAM A.A., MOSAVI DARKA S.S.. THE EFFECT OF HUMAN CUMULUS CELL- CONDITIONED MEDIUM ON MOUSE EMBRYO DEVELOPMENT IN VITRO. DANESHVAR MEDICINE[Internet]. 2006;13(63):75-80. Available from: https://sid.ir/paper/30885/en

IEEE: Copy

A. KARIMPOUR, A.A. NEZHAD MOGHADAM, and S.S. MOSAVI DARKA, “THE EFFECT OF HUMAN CUMULUS CELL- CONDITIONED MEDIUM ON MOUSE EMBRYO DEVELOPMENT IN VITRO,” DANESHVAR MEDICINE, vol. 13, no. 63, pp. 75–80, 2006, [Online]. Available: https://sid.ir/paper/30885/en

Related Journal Papers