THE EFFECT OF OUTER MEMBRANE PROTEIN ON MURINE NEUTROPHIL ACTIVITY

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MOHABATI MOBAREZ A.; ZAVARAN HOSSEINI A.; TABARAEI BAHMAN; BEHZADIANNEZHAD GH.; HOSSEINIDOUST S.R.; VOJGANI M.

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Journal: DANESHVAR MEDICINE Year:2001 | Volume:8 | Issue:35 Page(s): 67-72

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Title

THE EFFECT OF OUTER MEMBRANE PROTEIN ON MURINE NEUTROPHIL ACTIVITY

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Keywords

BORDETELLA PERTUSISQ4

NEUTROPHILQ4

CHEMILUMINESCENCEQ2

OMPQ4

WCVQ4

Abstract

BORDETELLA PERTUSIS, the bacteria which produces whooping cough, is an organism that demonstrates a variety of NEUTROPHIL inhibiting effect. Dual infections with pertusis and other agent (viral and bacteria), suggests the immunosuppressive effects of B. pertusis. Animal studies have shown the inhibition of chemo tactic activity of PMN by pertusis toxin.hemi luminescence is an indirect measure of activated oxygen metabolites related to the NEUTROPHIL oxidative burst including super oxide anion, hydroxyl radical and single oxygen.uter membrane protein was isolated from BORDETELLA PERTUSIS Tohama 1. Mild enzymatic extraction with nonionic detergent was used to minimize configurationally changes to protein during isolation. With this process intact semi purified OMP were obtained. Outer membrane complex was analyzed by SDS-PAGE and Coomasie blue and silver stain method.emale Balb/C mice (4-6 weeks old) were immunized interaperitonealy with OMC, OMP and killed whole cell with or without alhydrogel. One to four weeb after final immunization, the animal were killed and sterile peritoneal NEUTROPHIL harvested with heparin zed HBSS and luminal enhanced CHEMILUMINESCENCE’s was determined.uminol-enhanced CHEMILUMINESCENCE’s of NEUTROPHILes from animal immunized with OMC and OMP was significantly greater than (10 fold) NEUTROPHILs of animal immunized with whole killed bacteria

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APA: Copy

MOHABATI MOBAREZ, A., ZAVARAN HOSSEINI, A., TABARAEI, BAHMAN, BEHZADIANNEZHAD, GH., HOSSEINIDOUST, S.R., & VOJGANI, M.. (2001). THE EFFECT OF OUTER MEMBRANE PROTEIN ON MURINE NEUTROPHIL ACTIVITY. DANESHVAR MEDICINE, 8(35), 67-72. SID. https://sid.ir/paper/31096/en

Vancouver: Copy

MOHABATI MOBAREZ A., ZAVARAN HOSSEINI A., TABARAEI BAHMAN, BEHZADIANNEZHAD GH., HOSSEINIDOUST S.R., VOJGANI M.. THE EFFECT OF OUTER MEMBRANE PROTEIN ON MURINE NEUTROPHIL ACTIVITY. DANESHVAR MEDICINE[Internet]. 2001;8(35):67-72. Available from: https://sid.ir/paper/31096/en

IEEE: Copy

A. MOHABATI MOBAREZ, A. ZAVARAN HOSSEINI, BAHMAN TABARAEI, GH. BEHZADIANNEZHAD, S.R. HOSSEINIDOUST, and M. VOJGANI, “THE EFFECT OF OUTER MEMBRANE PROTEIN ON MURINE NEUTROPHIL ACTIVITY,” DANESHVAR MEDICINE, vol. 8, no. 35, pp. 67–72, 2001, [Online]. Available: https://sid.ir/paper/31096/en

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