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Information Journal Paper

Title

ADIPOCYTE DERIVED HORMONES GENE EXPRESSION, RESISTIN AND VISFATIN, IN AGS GASTRIC CANCER CELL LINE

Pages

  165-169

Abstract

 Background: Adipose tissue has characteristics of an endocrine organ which releases a number of adipocyte-specific factors, known as adipocytokines. It has recently suggested that adipocytokines might play a role in pathogenesis and progression of certain cancers, especially in GASTRIC CANCER. This study has managed to investigate endogenous and/or exogenous expression of VISFATIN and RESISTIN in GASTRIC CANCER cell line.Methods: Cell culture and semi-quantitative reverse transcription polymerase chain reaction has performed to measure mRNA and protein expression of RESISTIN and VISFATIN in GASTRIC CANCER cell lines. ELISA test has performed for cell lysate and supernatant of cell culture to measure RESISTIN and VISFATIN protein expression and secretion.Results: Human GASTRIC CANCER cell line (AGS cell line) has found to express VISFATIN mRNA and protein but RESISTIN mRNA and protein has not expressed.Conclusion: VISFATIN has expressed endogenously in AGS human GASTRIC CANCER cells. Conversely RESISTIN has no expression. The results of this study has suggested that expression of adipocytokine proteins in real samples, could be a biomarker for GASTRIC CANCER.

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    APA: Copy

    GORGIAN MOHAMMADI, MASUMEH, HEDAYATI, MEHDI, ZARGHAMI, NOSRATOLLAH, GHAEMMAGHAMI, SARA, & MOHADDES, MOJTABA. (2013). ADIPOCYTE DERIVED HORMONES GENE EXPRESSION, RESISTIN AND VISFATIN, IN AGS GASTRIC CANCER CELL LINE. INTERNATIONAL JOURNAL OF CANCER MANAGEMENT (IRANIAN JOURNAL OF CANCER PREVENTION), 6(3), 165-169. SID. https://sid.ir/paper/312593/en

    Vancouver: Copy

    GORGIAN MOHAMMADI MASUMEH, HEDAYATI MEHDI, ZARGHAMI NOSRATOLLAH, GHAEMMAGHAMI SARA, MOHADDES MOJTABA. ADIPOCYTE DERIVED HORMONES GENE EXPRESSION, RESISTIN AND VISFATIN, IN AGS GASTRIC CANCER CELL LINE. INTERNATIONAL JOURNAL OF CANCER MANAGEMENT (IRANIAN JOURNAL OF CANCER PREVENTION)[Internet]. 2013;6(3):165-169. Available from: https://sid.ir/paper/312593/en

    IEEE: Copy

    MASUMEH GORGIAN MOHAMMADI, MEHDI HEDAYATI, NOSRATOLLAH ZARGHAMI, SARA GHAEMMAGHAMI, and MOJTABA MOHADDES, “ADIPOCYTE DERIVED HORMONES GENE EXPRESSION, RESISTIN AND VISFATIN, IN AGS GASTRIC CANCER CELL LINE,” INTERNATIONAL JOURNAL OF CANCER MANAGEMENT (IRANIAN JOURNAL OF CANCER PREVENTION), vol. 6, no. 3, pp. 165–169, 2013, [Online]. Available: https://sid.ir/paper/312593/en

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