CLUSTER AND PRINCIPAL COMPONENT ANALYSIS OF HUMAN GLIOBLASTOMA MULTIFORME (GBM) TUMOR PROTEOME

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POOLADI MEHDI; REZAEI TAVIRANI MOSTAFA; HASHEMI MEHRDAD; HESAMI TACKALLOU SAEED; KHAGHANI RAZI ABAD SOLMAZ; MORADI AFSHIN; ZALI ALIREZA; MOUSAVI MASOUMEH; FIROZI DALVAND LEILA; RAKHSHAN AZADEH; ZAMANIAN AZODI MONA

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Journal Paper

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Journal: International Journal of Cancer Management Year:2014 | Volume:7 | Issue:2 Page(s): 87-95

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Title

CLUSTER AND PRINCIPAL COMPONENT ANALYSIS OF HUMAN GLIOBLASTOMA MULTIFORME (GBM) TUMOR PROTEOME

Author(s)

Keywords

GLIOBLASTOMA

GLIOMA

PROTEOMICS

CLUSTER

2DG ELECTROPHORESIS

Abstract

Background: GLIOBLASTOMA Multiforme (GBM) or grade IV astrocytoma is the most common and lethal adult malignant brain tumor. Several of the molecular alterations detected in GLIOMAs may have diagnostic and/or prognostic implications. PROTEOMICS has been widely applied in various areas of science, ranging from the deciphering of molecular pathogen nests of discuses.Methods: In this study proteins were extracted from the tumor and normal brain tissues and then the protein purity was evaluated by Bradford test and spectrophotometry. In this study, proteins were separated by 2-Dimensional Gel (2DG) electrophoresis method and the spots were then analyzed and compared using statistical data and specific software. Protein CLUSTERing analysis was performed on the list of proteins deemed significantly altered in GLIOBLASTOMA tumors (t-test and one-way ANOVA; P<0.05).Results: The 2D gel showed totally 876 spots. We reported, 172 spots were exhibited differently in expression level (fold>2) for GLIOBLASTOMA. On each analytical 2D gel, an average of 876 spots was observed. In this study, 188 spots exhibited up regulation of expression level, whereas the remaining 232 spots were decreased in GLIOBLASTOMA tumor relative to normal tissue. Results demonstrate that functional CLUSTERing (up and down regulated) and Principal Component Analysis (PCA) has considerable merits in aiding the interpretation of proteomic data.Conclusion: 2D gel electrophoresis is the core of PROTEOMICS which permitted the separation of thousands of proteins. High resolution 2DE can resolve up to 5,000 proteins simultaneously. Using CLUSTER analysis, we can also form groups of related variables, similar to what is practiced in factor analysis.

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APA: Copy

POOLADI, MEHDI, REZAEI TAVIRANI, MOSTAFA, HASHEMI, MEHRDAD, HESAMI TACKALLOU, SAEED, KHAGHANI RAZI ABAD, SOLMAZ, MORADI, AFSHIN, ZALI, ALIREZA, MOUSAVI, MASOUMEH, FIROZI DALVAND, LEILA, RAKHSHAN, AZADEH, & ZAMANIAN AZODI, MONA. (2014). CLUSTER AND PRINCIPAL COMPONENT ANALYSIS OF HUMAN GLIOBLASTOMA MULTIFORME (GBM) TUMOR PROTEOME. INTERNATIONAL JOURNAL OF CANCER MANAGEMENT (IRANIAN JOURNAL OF CANCER PREVENTION), 7(2), 87-95. SID. https://sid.ir/paper/312602/en

Vancouver: Copy

POOLADI MEHDI, REZAEI TAVIRANI MOSTAFA, HASHEMI MEHRDAD, HESAMI TACKALLOU SAEED, KHAGHANI RAZI ABAD SOLMAZ, MORADI AFSHIN, ZALI ALIREZA, MOUSAVI MASOUMEH, FIROZI DALVAND LEILA, RAKHSHAN AZADEH, ZAMANIAN AZODI MONA. CLUSTER AND PRINCIPAL COMPONENT ANALYSIS OF HUMAN GLIOBLASTOMA MULTIFORME (GBM) TUMOR PROTEOME. INTERNATIONAL JOURNAL OF CANCER MANAGEMENT (IRANIAN JOURNAL OF CANCER PREVENTION)[Internet]. 2014;7(2):87-95. Available from: https://sid.ir/paper/312602/en

IEEE: Copy

MEHDI POOLADI, MOSTAFA REZAEI TAVIRANI, MEHRDAD HASHEMI, SAEED HESAMI TACKALLOU, SOLMAZ KHAGHANI RAZI ABAD, AFSHIN MORADI, ALIREZA ZALI, MASOUMEH MOUSAVI, LEILA FIROZI DALVAND, AZADEH RAKHSHAN, and MONA ZAMANIAN AZODI, “CLUSTER AND PRINCIPAL COMPONENT ANALYSIS OF HUMAN GLIOBLASTOMA MULTIFORME (GBM) TUMOR PROTEOME,” INTERNATIONAL JOURNAL OF CANCER MANAGEMENT (IRANIAN JOURNAL OF CANCER PREVENTION), vol. 7, no. 2, pp. 87–95, 2014, [Online]. Available: https://sid.ir/paper/312602/en

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