مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

376
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

128
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

COMPARISON OF PCR-BASED DIAGNOSIS WITH CENTRIFUGED-BASED ENRICHMENT METHOD FOR DETECTION OF BORRELIAPERSICA IN ANIMAL BLOOD SAMPLES

Pages

  7-12

Abstract

 Background: The mainstay of DIAGNOSIS of RELAPSING FEVER (RF) is demonstration of the spirochetes in Giemsa-stained thick blood smears, but during non fever periods the bacteria are very scanty and rarely detected in blood smears by mi­cros­copy. This study is aimed to evaluate the sensitivity of different methods developed for detection of low-grade spi­ro­chetemia.Methods: Animal blood samples with low degrees of spirochetemia were tested with two PCRs and a nested PCR tar­get­ing flaB, GlpQ, and rrs genes. Also, a centrifuged-based ENRICHMENT METHOD and Giemsa staining were per­formed on blood samples with various degrees of spirochetemia.Results: The flaB-PCR and nested rrs-PCR turned positive with various degrees of spirochetemia including the blood samples that turned negative with dark-field microscopy. The GlpQ-PCR was positive as far as at least one spi­ro­chete was seen in 5-10 microscopic fields. The sensitivity of GlpQ-PCR increased when DNA from Buffy Coat Layer (BCL) was used as template. The centrifuged-based ENRICHMENT METHOD turned positive with as low concentra­tion as 50 bacteria/ml blood, while Giemsa thick staining detected bacteria with concentrations ³ 25000 bacteria/ml. Conclusion: Centrifuged-based ENRICHMENT METHOD appeared as much as 500-fold more sensitive than thick smears, which makes it even superior to some PCR assays. Due to simplicity and minimal laboratory requirements, this method can be considered a valuable tool for DIAGNOSIS of RF in rural health centers.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    NADDAF, SAIED REZA, KISHDEHI, M., & SIAVASHI, M.R.. (2011). COMPARISON OF PCR-BASED DIAGNOSIS WITH CENTRIFUGED-BASED ENRICHMENT METHOD FOR DETECTION OF BORRELIAPERSICA IN ANIMAL BLOOD SAMPLES. JOURNAL OF ARTHROPOD-BORNE DISEASES, 5(1), 7-12. SID. https://sid.ir/paper/312758/en

    Vancouver: Copy

    NADDAF SAIED REZA, KISHDEHI M., SIAVASHI M.R.. COMPARISON OF PCR-BASED DIAGNOSIS WITH CENTRIFUGED-BASED ENRICHMENT METHOD FOR DETECTION OF BORRELIAPERSICA IN ANIMAL BLOOD SAMPLES. JOURNAL OF ARTHROPOD-BORNE DISEASES[Internet]. 2011;5(1):7-12. Available from: https://sid.ir/paper/312758/en

    IEEE: Copy

    SAIED REZA NADDAF, M. KISHDEHI, and M.R. SIAVASHI, “COMPARISON OF PCR-BASED DIAGNOSIS WITH CENTRIFUGED-BASED ENRICHMENT METHOD FOR DETECTION OF BORRELIAPERSICA IN ANIMAL BLOOD SAMPLES,” JOURNAL OF ARTHROPOD-BORNE DISEASES, vol. 5, no. 1, pp. 7–12, 2011, [Online]. Available: https://sid.ir/paper/312758/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button