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Information Journal Paper

Title

PFCRTGENE IN PLASMODIUM FALCIPARUM FIELD ISOLATES FROM MUZAFFARGARH, PAKISTAN

Pages

  204-214

Abstract

 Background: The aim of the study was to identify the prevalence of different species of Plasmodium and haplotypes of PFCRT in Plasmodium falciparum from the selected area.Methods: Overall, 10, 372 blood films of suspected MALARIAl patients were examined microscopically from rural health center Sinawan, district Muzaffargarh, PAKISTAN from November 2008 to November 2010.P. falciparum positive samples (both whole blood and FTA blood spotted cards) were used for DNA extraction. Nested PCR was used to amplify the PFCRT (codon 72-76) gene fragment. Sequencing was carried out to find the haplotypes in the amplified fragment of PFCRT gene.Result: Over all slide positivity rate (SPR), P. vivax and P. falciparum positivity rate was 21.40 %, 19.37 % and 2.03% respectively. FTA blood spotted cards were equally efficient in the blood storage for PCR and sequencing.Analysis of sequencing results of PFCRT showed only one type of haplotype SagtVMNT (AGTGTAATGAATACA) from codon 72-76 in all samples.Conclusion: The results show high prevalence of CQ RESISTANCE and AQ resistant genes. AQ is not recommended to be used as a partner drug in ACT in this locality, so as to ward off future catastrophes.

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    APA: Copy

    SAHAR, SUMRIN, AKHTAR, TANVEER, ALI, AKBAR, BILAL, HAZRAT, & SALEEM, RANA MUHAMMAD. (2015). PFCRTGENE IN PLASMODIUM FALCIPARUM FIELD ISOLATES FROM MUZAFFARGARH, PAKISTAN. JOURNAL OF ARTHROPOD-BORNE DISEASES, 9(2), 204-214. SID. https://sid.ir/paper/312855/en

    Vancouver: Copy

    SAHAR SUMRIN, AKHTAR TANVEER, ALI AKBAR, BILAL HAZRAT, SALEEM RANA MUHAMMAD. PFCRTGENE IN PLASMODIUM FALCIPARUM FIELD ISOLATES FROM MUZAFFARGARH, PAKISTAN. JOURNAL OF ARTHROPOD-BORNE DISEASES[Internet]. 2015;9(2):204-214. Available from: https://sid.ir/paper/312855/en

    IEEE: Copy

    SUMRIN SAHAR, TANVEER AKHTAR, AKBAR ALI, HAZRAT BILAL, and RANA MUHAMMAD SALEEM, “PFCRTGENE IN PLASMODIUM FALCIPARUM FIELD ISOLATES FROM MUZAFFARGARH, PAKISTAN,” JOURNAL OF ARTHROPOD-BORNE DISEASES, vol. 9, no. 2, pp. 204–214, 2015, [Online]. Available: https://sid.ir/paper/312855/en

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