MIR-155 DOWN REGULATION BY LNA INHIBITOR CAN REDUCE CELL GROWTH AND PROLIFERATION IN PC12 CELL LINE

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KOUHKAN FATEMEH; ALIZADEH SHABAN; KAVIANI SAEID; SOLEIMANI MASOUD; POURFATHOLLAH ALI AKBAR; AMIRIZADEH NASER; ABROUN SAEID; NORUZINIA MEHRDAD; MOHAMADI SHAHIN

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Journal: Avicenna Journal of Medical Biotechnology Year:2011 | Volume:3 | Issue:2 (9) Page(s): 61-66

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Title

MIR-155 DOWN REGULATION BY LNA INHIBITOR CAN REDUCE CELL GROWTH AND PROLIFERATION IN PC12 CELL LINE

Author(s)

Keywords

APOPTOSIS

MIR-155

MTT TEST

PC12 CELLS

Abstract

MicroRNAs (miRNAs) are a class of small non coding regulatory RNAs that have key functions in multiple cell processes. Deregulation of these tiny miRNAs is involved in various human diseases. MIR-155 is one of the multifunctional miRNA that its over-expression has been found to be associated with different kinds of cancer such as leukemia, breast and colon cancers. It is thought that deregulation and over-expression of this microRNA may be associated with PC12 cell proliferation. So, the aim of this study was to investigate the role of MIR-155 expression on PC12 cell growth. For this reason, PC12 CELLS were cultured and transfected by 3 different concentration (25, 50 and 75nmol) of either LNA anti-miR-155 or scramble antisense in 24-well plate. Then, total RNA was extracted from transfected cells. miRNA cDNAs were synthesized from isolated total RNA. In the second step, MIR-155 expression level was analyzed using the quantitative real-time polymerase chain reaction (QRT-PCR). MTT TEST was performed to evaluate cell viability.In the next step, APOPTOSIS assay was assessed to investigate anti MIR-155 effect on PC12 CELLS death. Obtained results were analyzed with t-test. MTT TEST revealed that cell viability of transfected cells with 75nM of anti-miR- 155 to be reduced by half of the control and scramble groups (0.5 vs.0.97 and 0.94). Our data suggest that MIR-155 over-expression is associated with PC12 cell growth. So, MIR-155 down regulation by anti-miR-155 could open up new ways to restrain brain tumor growth, as anti-miR-155 causes PC12 CELLS to repress.

Cites

اثر یک دوره تمرین استقامتی بر بیان mir-155 و بیان ژن SOCS1 در تومور موش های ماده مبتلا به سرطان پستان

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APA: Copy

KOUHKAN, FATEMEH, ALIZADEH, SHABAN, KAVIANI, SAEID, SOLEIMANI, MASOUD, POURFATHOLLAH, ALI AKBAR, AMIRIZADEH, NASER, ABROUN, SAEID, NORUZINIA, MEHRDAD, & MOHAMADI, SHAHIN. (2011). MIR-155 DOWN REGULATION BY LNA INHIBITOR CAN REDUCE CELL GROWTH AND PROLIFERATION IN PC12 CELL LINE. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 3(2 (9)), 61-66. SID. https://sid.ir/paper/313755/en

Vancouver: Copy

KOUHKAN FATEMEH, ALIZADEH SHABAN, KAVIANI SAEID, SOLEIMANI MASOUD, POURFATHOLLAH ALI AKBAR, AMIRIZADEH NASER, ABROUN SAEID, NORUZINIA MEHRDAD, MOHAMADI SHAHIN. MIR-155 DOWN REGULATION BY LNA INHIBITOR CAN REDUCE CELL GROWTH AND PROLIFERATION IN PC12 CELL LINE. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2011;3(2 (9)):61-66. Available from: https://sid.ir/paper/313755/en

IEEE: Copy

FATEMEH KOUHKAN, SHABAN ALIZADEH, SAEID KAVIANI, MASOUD SOLEIMANI, ALI AKBAR POURFATHOLLAH, NASER AMIRIZADEH, SAEID ABROUN, MEHRDAD NORUZINIA, and SHAHIN MOHAMADI, “MIR-155 DOWN REGULATION BY LNA INHIBITOR CAN REDUCE CELL GROWTH AND PROLIFERATION IN PC12 CELL LINE,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 3, no. 2 (9), pp. 61–66, 2011, [Online]. Available: https://sid.ir/paper/313755/en

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