FETAL SEX DETERMINATION USING NON-INVASIVE METHOD OF CELL-FREE FETAL DNA IN MATERNAL PLASMA OF PREGNANT WOMEN DURING 6TH-10TH WEEKS OF GESTATION

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ZARGARI MARYAM; SADEGHI MOHAMMAD REZA; SHAHHOSSEINY MOHAMMAD HASSAN; KAMALI KOROUSH; SALIMINEJAD KYOMARS; ESMAEILZADEH ALI; KHORRAM KHORSHID HAMID REZA

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Journal: Avicenna Journal of Medical Biotechnology Year:2011 | Volume:3 | Issue:4 (11) Page(s): 201-206

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Title

FETAL SEX DETERMINATION USING NON-INVASIVE METHOD OF CELL-FREE FETAL DNA IN MATERNAL PLASMA OF PREGNANT WOMEN DURING 6TH-10TH WEEKS OF GESTATION

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Keywords

FETUS

GENETIC MATERIAL

PRENATAL DIAGNOSIS

SEX DETERMINATION

Abstract

In previous years, identification of fetal cells in maternal blood circulation has caused a new revolution in non-invasive method of PRENATAL DIAGNOSIS. Low number of fetal cells in maternal blood and long-term survival after pregnancy limited the use of fetal cells in diagnostic and clinical applications. With the discovery of cell-free fetal DNA (cffDNA) in plasma of pregnant women, access to GENETIC MATERIAL of the FETUS had become possible to determine early gender of a FETUS in pregnancies at the risk of X-linked genetic conditions instead of applying invasive methods. Therefore in this study, the probability of detecting sequences on the Y chromosome in pregnant women has been evaluated to identify the gender of FETUSes. Peripheral blood samples were obtained from 80 pregnant women at 6th to 10th weeks of gestation and then the fetal DNA was extracted from the plasma. Nested PCR was applied to detect the sequences of single copy SRY gene and multi copy DYS14 & DAZ genes on the Y chromosome of the male FETUSes. At the end, all the obtained results were compared with the actual gender of the newborns. In 40 out of 42 born baby boys, the relevant gene sequences were identified and 95.2% sensitivity was obtained. Non-invasive early determination of fetal gender using cffDNA could be employed as a pre-test in the shortest possible time and with a high reliability to avoid applying invasive methods in cases where a FETUS is at the risk of genetic diseases.

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APA: Copy

ZARGARI, MARYAM, SADEGHI, MOHAMMAD REZA, SHAHHOSSEINY, MOHAMMAD HASSAN, KAMALI, KOROUSH, SALIMINEJAD, KYOMARS, ESMAEILZADEH, ALI, & KHORRAM KHORSHID, HAMID REZA. (2011). FETAL SEX DETERMINATION USING NON-INVASIVE METHOD OF CELL-FREE FETAL DNA IN MATERNAL PLASMA OF PREGNANT WOMEN DURING 6^TH-10^TH WEEKS OF GESTATION. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 3(4 (11)), 201-206. SID. https://sid.ir/paper/313767/en

Vancouver: Copy

ZARGARI MARYAM, SADEGHI MOHAMMAD REZA, SHAHHOSSEINY MOHAMMAD HASSAN, KAMALI KOROUSH, SALIMINEJAD KYOMARS, ESMAEILZADEH ALI, KHORRAM KHORSHID HAMID REZA. FETAL SEX DETERMINATION USING NON-INVASIVE METHOD OF CELL-FREE FETAL DNA IN MATERNAL PLASMA OF PREGNANT WOMEN DURING 6^TH-10^TH WEEKS OF GESTATION. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2011;3(4 (11)):201-206. Available from: https://sid.ir/paper/313767/en

IEEE: Copy

MARYAM ZARGARI, MOHAMMAD REZA SADEGHI, MOHAMMAD HASSAN SHAHHOSSEINY, KOROUSH KAMALI, KYOMARS SALIMINEJAD, ALI ESMAEILZADEH, and HAMID REZA KHORRAM KHORSHID, “FETAL SEX DETERMINATION USING NON-INVASIVE METHOD OF CELL-FREE FETAL DNA IN MATERNAL PLASMA OF PREGNANT WOMEN DURING 6^TH-10^TH WEEKS OF GESTATION,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 3, no. 4 (11), pp. 201–206, 2011, [Online]. Available: https://sid.ir/paper/313767/en

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