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Information Journal Paper

Title

GENOTYPING OF POLYMORPHIC MICROSATELLITE MARKERS LINKED TO RB1 LOCUS IN IRANIAN POPULATION

Pages

  193-199

Abstract

 Background: RETINOBLASTOMA is the most common intraocular tumor in childhood and mutation in theRB1 gene will trigger the tumorigenesis. So far, a wide range of the mutations along the length of RB1 gene have been reported.However, some could not be detected by common detection methods. In such condition, linkage analysis using microsatellite markers is suggested to trace unknownRB1 mutations in the affected family. The aim of the present study was to evaluate the heterozygosity rates and genotyping of three microsatellite markers near or inside of theRB1 gene.Methods: Totally, 120 unrelated healthy people from Fardis, Karaj, IRAN were recruited and from each participant genomic DNA was extracted from 5 ml of peripheral blood. Three microsatellite markers D13S153, D13S156 and D13S128 located within or adjacent to theRB1 gene were selected for linkage analysis. The reliability of microsatellite markers and linkage analysis were investigated in 10 members of 2 families with familial RETINOBLASTOMA.Results: Our results showed that heterozygosity rates for the three markers D13S153, D13S156 and D13S128 were 74, 70 and 78%, respectively. On the other hand, 2 and 36 out of 120 people were homozygote and heterozygous for all loci, respectively.Conclusion: Given the heterozygosity rates, it may be concluded that all microsatellite markers D13S153, D13S156 and D13S128 are informative and have a high rate of heterozygosity and sensitivity. Therefore, tracing the unknown RB1mutated alleles using linkage analysis in IRANian family with familial RETINOBLASTOMA could be recommended by means of these three microsatellite markers.

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    APA: Copy

    MOHAMAD ZAHERY, SAMAN, SALIMINEJAD, KIOOMARS, KHORRAM KHORSHID, HAMID REZA, & AHANI, ALI. (2012). GENOTYPING OF POLYMORPHIC MICROSATELLITE MARKERS LINKED TO RB1 LOCUS IN IRANIAN POPULATION. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 4(4 (15)), 193-199. SID. https://sid.ir/paper/313793/en

    Vancouver: Copy

    MOHAMAD ZAHERY SAMAN, SALIMINEJAD KIOOMARS, KHORRAM KHORSHID HAMID REZA, AHANI ALI. GENOTYPING OF POLYMORPHIC MICROSATELLITE MARKERS LINKED TO RB1 LOCUS IN IRANIAN POPULATION. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2012;4(4 (15)):193-199. Available from: https://sid.ir/paper/313793/en

    IEEE: Copy

    SAMAN MOHAMAD ZAHERY, KIOOMARS SALIMINEJAD, HAMID REZA KHORRAM KHORSHID, and ALI AHANI, “GENOTYPING OF POLYMORPHIC MICROSATELLITE MARKERS LINKED TO RB1 LOCUS IN IRANIAN POPULATION,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 4, no. 4 (15), pp. 193–199, 2012, [Online]. Available: https://sid.ir/paper/313793/en

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