مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Cites:

1

Information Journal Paper

Title

PRODUCTION AND CHARACTERIZATION OF A MURINE MONOCLONAL ANTIBODY AGAINST HUMAN FERRITIN

Pages

  212-219

Abstract

 Background: FERRITIN is an iron storage protein, which plays a key role in iron metabolism. Measurement of FERRITIN level in serum is one of the most useful indicators of iron status and also a sensitive measurement of iron deficiency.Monoclonal antibodies may be useful as a tool in various aspects of FERRITIN investigations.In this paper, the production of a murine MONOCLONAL ANTIBODY (mAb) against human FERRITIN was reported.Methods: Balb/c mice were immunized with purified human FERRITIN and splenocytes of hyper immunized mice were fused with Sp2/0 myeloma cells.After four times of cloning by limiting dilution, a positive hybridoma (clone: 2F9-C9) was selected by ELISA using human FERRITIN. Anti-ferritin mAb was purified from culture supernatants by affinity chromatography.Results: Determination of the antibody affinity for FERRITIN by ELISA revealed a relatively high affinity (2.34×109 M-1) and the isotype was determined to be IgG2a. The anti-ferritin mAb 2F9-C9 reacted with 79.4% of Hela cells in FLOW CYTOMETRY. The antibody detected a band of 20kDa in K562 cells, murine and human liver lysates, purified FERRITIN in Western blot and also FERRITIN in human serum.Conclusion: This mAb can specifically recognize FERRITIN and may serve as a component of FERRITIN diagnostic kit if other requirements of the kit are met.

Cites

References

Cite

APA: Copy

BAYAT, ALI AHMAD, OMID YEGANEH, N., GHODS, ROYA, ZARNANI, AMIR HASSAN, BAHJATI ARDEKANI, REZA, MAHMOUDI, AHMAD REZA, MAHMOUDIAN, JAFAR, HAGHIGHAT NOUTASH, FARZANEH, & JEDDI TEHRANI, MAHMOOD. (2013). PRODUCTION AND CHARACTERIZATION OF A MURINE MONOCLONAL ANTIBODY AGAINST HUMAN FERRITIN. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 5(4 (18)), 212-219. SID. https://sid.ir/paper/313820/en

Vancouver: Copy

BAYAT ALI AHMAD, OMID YEGANEH N., GHODS ROYA, ZARNANI AMIR HASSAN, BAHJATI ARDEKANI REZA, MAHMOUDI AHMAD REZA, MAHMOUDIAN JAFAR, HAGHIGHAT NOUTASH FARZANEH, JEDDI TEHRANI MAHMOOD. PRODUCTION AND CHARACTERIZATION OF A MURINE MONOCLONAL ANTIBODY AGAINST HUMAN FERRITIN. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2013;5(4 (18)):212-219. Available from: https://sid.ir/paper/313820/en

IEEE: Copy

ALI AHMAD BAYAT, N. OMID YEGANEH, ROYA GHODS, AMIR HASSAN ZARNANI, REZA BAHJATI ARDEKANI, AHMAD REZA MAHMOUDI, JAFAR MAHMOUDIAN, FARZANEH HAGHIGHAT NOUTASH, and MAHMOOD JEDDI TEHRANI, “PRODUCTION AND CHARACTERIZATION OF A MURINE MONOCLONAL ANTIBODY AGAINST HUMAN FERRITIN,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 5, no. 4 (18), pp. 212–219, 2013, [Online]. Available: https://sid.ir/paper/313820/en

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