مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

DOWNREGULATION OF MMP2 AND BCL-2 IN ADIPOSE DERIVED STEM CELLS (ASCS) FOLLOWING TRANSFECTION WITH IP-10 GENE

Pages

  27-37

Abstract

 Background: Mesenchymal Stem Cells (MSCs) are recently introduced as novel immunological gene carriers for treatment of cancer. It is believed that balance between the expression of angiogenic and anti-angiogenic factors, such as SDF-1 and IP-10, may regulate neovascularization within the tumor.Methods: In this study, we compared the expression of important tumor pro-moting mediators in IP-10-transfected ADIPOSE DERIVED STEM CELLS (ASCs) to those transfected with SDF-1. ASCs were isolated from adipose tissue of a normal subject undergoing cosmetic mamoplasty surgery using collagenase. ASCs were transfected with IP-10 or SDF-1 propagated plasmids by electroporation method and Lipofectamin 2000. Expressions of SDF-1, CXCR4, IP-10, Bcl-2, MMP2, IL-10, IGF-1, and VEGF were detected in transfected ASCs using quantitative realReal-time Time polymerase Polymerase chain Chain reaction Reaction (qRT-PCR).Results: Results showed that the expressions of SDF-1, CXCR4, Bcl-2, MMP2, IL-10, IGF-1, and VEGF were upregulated in SDF-1-transfected ASCs. In contrast, Bcl-2 and MMP2 transcripts showed 45×103- and 10- fold lower expression in ASCs transfected with IP-10 compared to non-transfected cells.Conclusion: Anti-angiogenic chemokines such as IP-10 may modulate tumor promoting properties of ASCs and would be introduced as novel candidates for TUMOR IMMUNOTHERAPY; however, further studies are needed to be conducted.

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    APA: Copy

    RAZMKHAH, MAHBOOBEH, JABERIPOUR, MANSOOREH, & GHADERI, ABBAS. (2014). DOWNREGULATION OF MMP2 AND BCL-2 IN ADIPOSE DERIVED STEM CELLS (ASCS) FOLLOWING TRANSFECTION WITH IP-10 GENE. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 6(1), 27-37. SID. https://sid.ir/paper/313825/en

    Vancouver: Copy

    RAZMKHAH MAHBOOBEH, JABERIPOUR MANSOOREH, GHADERI ABBAS. DOWNREGULATION OF MMP2 AND BCL-2 IN ADIPOSE DERIVED STEM CELLS (ASCS) FOLLOWING TRANSFECTION WITH IP-10 GENE. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2014;6(1):27-37. Available from: https://sid.ir/paper/313825/en

    IEEE: Copy

    MAHBOOBEH RAZMKHAH, MANSOOREH JABERIPOUR, and ABBAS GHADERI, “DOWNREGULATION OF MMP2 AND BCL-2 IN ADIPOSE DERIVED STEM CELLS (ASCS) FOLLOWING TRANSFECTION WITH IP-10 GENE,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 6, no. 1, pp. 27–37, 2014, [Online]. Available: https://sid.ir/paper/313825/en

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