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Information Journal Paper

Title

EVALUATION OF A MODIFIED DNA EXTRACTION METHOD FOR ISOLATION OF CELL-FREE FETAL DNA FROM MATERNAL SERUM (SHORT COMMUNICATION)

Pages

  85-88

Abstract

 Background: Discovery of short cell free fetal DNA (cffDNA) fragments in maternal plasma has created major changes in the field of PRENATAL DIAGNOSIS. The use of cffDNA to set up noninvasive prenatal test is limited due to the low concentration of fetal DNA in maternal plasma therefore, employing a high efficiency extraction method leads to more accurate results. The aim of this study was to evaluate the efficiency of Triton/Heat/Phenol (THP) protocol in comparison with the QIAamp DNA Blood mini Kit for cffDNA purification.Methods: In order to evaluate the efficiency of THP PROTOCOL, DNA of Rhesus D (RhD) negative pregnant women's plasma was collected, then real-time PCR for RHD exon 7 was performed. The Ct value data of real time PCR obtained by two different methods were compared and after delivery serology test on cord blood was done to validate the real time PCR results.Results: The results indicated significant differences between two extraction methods (p=0.001). The mean±SD of Ct-value using THP PROTOCOL was 33.8±1.6 and 36.1±2.47 using QIAamp DNA Blood mini Kit.Conclusion: our finding demonstrated that THP PROTOCOL was more effective than the QIAamp DNA Blood mini Kits for cffDNA extraction and lead to decrease the false negative results.

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    APA: Copy

    KESHAVARZ, ZEINAB, MOEZZI, LEILI, RANJBARAN, REZA, ABOUALIZADEH, FARZANEH, BEHZAD BEHBAHANI, ABBAS, ABDULLAHI, MASOOMA, & SHARIFZADEH, SEDIGHEH. (2015). EVALUATION OF A MODIFIED DNA EXTRACTION METHOD FOR ISOLATION OF CELL-FREE FETAL DNA FROM MATERNAL SERUM (SHORT COMMUNICATION). AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 7(2), 85-88. SID. https://sid.ir/paper/313871/en

    Vancouver: Copy

    KESHAVARZ ZEINAB, MOEZZI LEILI, RANJBARAN REZA, ABOUALIZADEH FARZANEH, BEHZAD BEHBAHANI ABBAS, ABDULLAHI MASOOMA, SHARIFZADEH SEDIGHEH. EVALUATION OF A MODIFIED DNA EXTRACTION METHOD FOR ISOLATION OF CELL-FREE FETAL DNA FROM MATERNAL SERUM (SHORT COMMUNICATION). AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2015;7(2):85-88. Available from: https://sid.ir/paper/313871/en

    IEEE: Copy

    ZEINAB KESHAVARZ, LEILI MOEZZI, REZA RANJBARAN, FARZANEH ABOUALIZADEH, ABBAS BEHZAD BEHBAHANI, MASOOMA ABDULLAHI, and SEDIGHEH SHARIFZADEH, “EVALUATION OF A MODIFIED DNA EXTRACTION METHOD FOR ISOLATION OF CELL-FREE FETAL DNA FROM MATERNAL SERUM (SHORT COMMUNICATION),” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 7, no. 2, pp. 85–88, 2015, [Online]. Available: https://sid.ir/paper/313871/en

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