SHORT COMMUNICATION: CONSTRUCTION OF CTLA-4-IG FUSION GENE IN PBUDCE4.1 EXPRESSION VECTOR

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YAZDANPANAH SAMANI MAHSA; MAHMOUDI MAYMAND ELHAM; JAHANGEERFAM TAYEBEH; GHADERI ABBAS

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Journal Paper

Paper Information

Journal: Avicenna Journal of Medical Biotechnology Year:2015 | Volume:7 | Issue:4 (27) Page(s): 179-181

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Title

SHORT COMMUNICATION: CONSTRUCTION OF CTLA-4-IG FUSION GENE IN PBUDCE4.1 EXPRESSION VECTOR

Author(s)

YAZDANPANAH SAMANI MAHSA | MAHMOUDI MAYMAND ELHAM | JAHANGEERFAM TAYEBEH | GHADERI ABBAS | Issue Writer Certificate

Keywords

ABATACEPT

CTLA-4 ANTIGEN

CTLA4-IG

RECOMBINANT DNA

Abstract

Background: CTLA-4 inhibitory signals prevent cell cycle progression and IL-2 production, leading to a halt on an ongoing immune response. CTLA4-IG fusion proteins contain the extracellular domain of CTLA-4 and Fc fragment of human IgG antibody. In this study we aimed to fuse the ctla-4 gene encoding the extracellular domain of CTLA-4 molecule with igg1 gene encoding Fc region of human IgG.Methods: After primer design, PCR reaction was performed using pfu polymerase enzyme and specific primers. PCR amplified fragment was ligated into the vector containing the human igg1 gene. The resulting fusion fragment of ctla-4 and human igg1 genes was ligated to pBudCE4.1 expression vector.Results: Extracellular domain of ctla-4 gene was ligated to igg1 gene and then CTLA4-IG fragment was cloned into pBudCE4.1 vector. Construction of the expression vector was confirmed by restriction pattern analysis and sequencing.Conclusion: By confirming the construct, in the next step, the RECOMBINANT DNA will be used to produce CTLA4-IG recombinant protein for the clinical uses.

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APA: Copy

YAZDANPANAH SAMANI, MAHSA, MAHMOUDI MAYMAND, ELHAM, JAHANGEERFAM, TAYEBEH, & GHADERI, ABBAS. (2015). SHORT COMMUNICATION: CONSTRUCTION OF CTLA-4-IG FUSION GENE IN PBUDCE4.1 EXPRESSION VECTOR. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 7(4 (27)), 179-181. SID. https://sid.ir/paper/313886/en

Vancouver: Copy

YAZDANPANAH SAMANI MAHSA, MAHMOUDI MAYMAND ELHAM, JAHANGEERFAM TAYEBEH, GHADERI ABBAS. SHORT COMMUNICATION: CONSTRUCTION OF CTLA-4-IG FUSION GENE IN PBUDCE4.1 EXPRESSION VECTOR. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2015;7(4 (27)):179-181. Available from: https://sid.ir/paper/313886/en

IEEE: Copy

MAHSA YAZDANPANAH SAMANI, ELHAM MAHMOUDI MAYMAND, TAYEBEH JAHANGEERFAM, and ABBAS GHADERI, “SHORT COMMUNICATION: CONSTRUCTION OF CTLA-4-IG FUSION GENE IN PBUDCE4.1 EXPRESSION VECTOR,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 7, no. 4 (27), pp. 179–181, 2015, [Online]. Available: https://sid.ir/paper/313886/en

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