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Information Journal Paper

Title

THE EFFECT OF MEDIA SUPPLEMENTATION WITH ANGIOTENSIN ON DEVELOPMENTAL COMPETENCE OF OVINE EMBRYOS DERIVED FROM VITRIFIED-WARMED OOCYTES

Pages

  139-144

Keywords

NA+/K+/ATPASE 

Abstract

 Background: This study was aimed to assess the effects of ANGIOTENSIN II (Ang II) supplementation to the In Vitro Maturation (IVM) and In Vitro Culture (IVC) media of vitrified-warmed OVINE OOCYTES on their developmental competence and expression of Na+/K+/ATPase in resulting embryos.Methods: The slaughterhouse-derived immature OOCYTES (n=1069) were randomly distributed into four experimental groups: groups I and II) IVM/IVF and IVC of fresh and vitrified OOCYTES without angiotensin supplementation (Control-Fresh and Control-Vit groups, respectively); group III) IVM of vitrified OOCYTES in the presence of Ang II followed by IVF/IVC (Vit-IVM group); and group IV) IVM/IVF of vitrified OOCYTES followed by IVC wherein the embryos were exposed to Ang II on day 4 of IVC (Vit-D4 group).The embryos were immunostained with primary antibodies against Na+/K+/ATPase a1 and b1 subunits.Results: In Vit-IVM and Vit-D4 groups, the rates of expanded and total blastocysts on day 7 as well as the proportion of blastocysts on day 8 were increased. The expression of Na+/K+/ATPase a1 and b 1 subunits were positively influenced by the addition of Ang II on day 4 (Vit-D4 group).Conclusion: The addition of Ang II to the IVM and IVC media could improve blastocysts formation in vitrified sheep OOCYTES. This improvement might be related to the greater expression of Na+/K+/ATPase a1 and b 1 subunits when Ang II was added during IVC.

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    APA: Copy

    NADERI, MOHAMMAD MEHDI, BORJIAN BOROUJENI, SARA, SARVARI, ALI, HEYDARI, BANAFSHEH, AKHONDI, MOHAMMAD MEHDI, ZARNANI, AMIR HASSAN, & SHIRAZI, ABOLFAZL. (2016). THE EFFECT OF MEDIA SUPPLEMENTATION WITH ANGIOTENSIN ON DEVELOPMENTAL COMPETENCE OF OVINE EMBRYOS DERIVED FROM VITRIFIED-WARMED OOCYTES. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 8(3), 139-144. SID. https://sid.ir/paper/313893/en

    Vancouver: Copy

    NADERI MOHAMMAD MEHDI, BORJIAN BOROUJENI SARA, SARVARI ALI, HEYDARI BANAFSHEH, AKHONDI MOHAMMAD MEHDI, ZARNANI AMIR HASSAN, SHIRAZI ABOLFAZL. THE EFFECT OF MEDIA SUPPLEMENTATION WITH ANGIOTENSIN ON DEVELOPMENTAL COMPETENCE OF OVINE EMBRYOS DERIVED FROM VITRIFIED-WARMED OOCYTES. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2016;8(3):139-144. Available from: https://sid.ir/paper/313893/en

    IEEE: Copy

    MOHAMMAD MEHDI NADERI, SARA BORJIAN BOROUJENI, ALI SARVARI, BANAFSHEH HEYDARI, MOHAMMAD MEHDI AKHONDI, AMIR HASSAN ZARNANI, and ABOLFAZL SHIRAZI, “THE EFFECT OF MEDIA SUPPLEMENTATION WITH ANGIOTENSIN ON DEVELOPMENTAL COMPETENCE OF OVINE EMBRYOS DERIVED FROM VITRIFIED-WARMED OOCYTES,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 8, no. 3, pp. 139–144, 2016, [Online]. Available: https://sid.ir/paper/313893/en

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