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Information Journal Paper

Title

INDUCTION OF EPIGENETIC ALTERATION BY CPUK02, AN ENT- KAURENOID DERIVATIVE OF STEVIOSIDE

Pages

  13-18

Abstract

 Background: Dietary polyphenols, such as those found in green tea and red wine, are linked to antitumor activity. They are known to influence many signaling pathways EPIGENETICally within the human body. In this regard, CPUK02 (15-Oxosteviol benzyl ester) is a new ent-kaurenoid derivative of stevioside and exhibits strong anti-cancer activityin vitro and in vivo. Nowadays, the role of EPIGENETICs in cancer has been the subject of intensive study and DNA METHYLATION targeting represents a relevant strategy for cancer treatment. There are no reports regarding the effects of CPUK02 on EPIGENETIC alterations in colorectal cancer cell line. This study was an attempt to compare CPUK02 with 5-AZA as DNMT inhibitor agent and evaluate whether it can induce its anti-cancer effects via altering the level of DNMT3b mRNA, MGMT and SFRP2 METHYLATION pattern in HCT 116 cell line.Methods: To evaluate DNMT3b expression, DNMT3B mRNA levels in HCT116 CRC cell line were quantified by real-time reverse-transcriptase Polymerase Chain Reaction (PCR) assay after 24hr of incubation time with CPUK02 and 5-AZA. In addition, the METHYLATION patterns of 2 CpG islands in this cell line were examined by METHYLATIONspecific PCR methods.Results: CPUK02 surprisingly, decreased the DNMT3b mRNA level. The average expression levels of DNMT3b in HCT116 treated with CPUK02 and 5-AZA relative to the GAPDH expression level in control were 0.16 and 0.5%, respectively. Furthermore, CPUK02 could decrease the methylated allele of MGMT and SFRP2 genes in HCT 116 after 24hr.Conclusion: In this study, positive correlation was found between mRNA expression of DNMT3b and gene promoter hypermethylation after treatment with CPUK02 and 5-AZA. Our data confirmed that CPUK02 like 5-AZA exhibits demethylating properties.

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    Cite

    APA: Copy

    MOKARRAM, POONEH, MOHAMMADI, ZEINAB, KHAZAYEL, SAEID, & DAYONG, ZHANG. (2017). INDUCTION OF EPIGENETIC ALTERATION BY CPUK02, AN ENT- KAURENOID DERIVATIVE OF STEVIOSIDE. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 9(1), 13-18. SID. https://sid.ir/paper/313928/en

    Vancouver: Copy

    MOKARRAM POONEH, MOHAMMADI ZEINAB, KHAZAYEL SAEID, DAYONG ZHANG. INDUCTION OF EPIGENETIC ALTERATION BY CPUK02, AN ENT- KAURENOID DERIVATIVE OF STEVIOSIDE. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2017;9(1):13-18. Available from: https://sid.ir/paper/313928/en

    IEEE: Copy

    POONEH MOKARRAM, ZEINAB MOHAMMADI, SAEID KHAZAYEL, and ZHANG DAYONG, “INDUCTION OF EPIGENETIC ALTERATION BY CPUK02, AN ENT- KAURENOID DERIVATIVE OF STEVIOSIDE,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 9, no. 1, pp. 13–18, 2017, [Online]. Available: https://sid.ir/paper/313928/en

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