EVALUATION OF IMMUNE RESPONSES INDUCED BY GRA7 AND ROP2 GENES BY DNA VACCINE COCKTAILS AGAINST ACUTE TOXOPLASMOSIS IN BALB/C MICE

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Vazini Hossein; GHAFARIFAR FATEMEH; SHARIFI ZOHREH; Dalimi Abdolhosein

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Journal: Avicenna Journal of Medical Biotechnology Year:2018 | Volume:10 | Issue:1 Page(s): 2-8

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Title

EVALUATION OF IMMUNE RESPONSES INDUCED BY GRA7 AND ROP2 GENES BY DNA VACCINE COCKTAILS AGAINST ACUTE TOXOPLASMOSIS IN BALB/C MICE

Author(s)

Vazini Hossein | GHAFARIFAR FATEMEH | SHARIFI ZOHREH | Dalimi Abdolhosein | Issue Writer Certificate

Keywords

BALB/C

DNA VACCINE

GRA7

ROP2

TOXOPLASMA GONDII

Abstract

Background: The severe damages of toxoplasmosis clearly indicate the need for the development of a more effective vaccine. Immunization with plasmid DNA is a promising vaccination technique. Therefore, GRA7 plasmid was prepared to be used as a vaccine. The purpose of this study was evaluation of immunization with cocktail DNA VACCINE including plasmids encoding TOXOPLASMA GONDII ROP2 and GRA7 in BALB/C mice.Methods: In this study, 733 bp of GRA7 gene was cloned in pCDNA3.1 plasmid as an expression vector. The plasmids containingGRA7 and ROP2 genes were administered via IMaccording to immunized mice three times with a 3 week interval. For lymphocyte proliferation and cytokine assay, splenocytes of immunized mice were cultured for proliferation and cytokine assay. The other mice in each group were inoculated by the parasite and mortality of the mice was evaluated on a daily basis.Results: The cytokine assay results and lymphocyte proliferation response in cocktail DNA VACCINEs showed that IFN-γ levels were significantly higher than controls (p<0.05), whereas IL-4 expression level in BALB/C mice immunized with cocktail was lower than that in control groups and these results are confirmed by MTT test. Predominance of the levels of IgG2a over IgG1 was observed in sera of the immunized mice. Furthermore, IgG2a values in cocktail DNA VACCINEs pcGRA7 were significantly higher than control group (p<0.01). In contrast, IgG1 antibodies were similar between the two groups (p>0.5). So, survival time in the immune groups was significantly prolonged in comparison to control ones (p<0.01).Conclusion: The immunized mice by DNA VACCINE produce higher titration of IFNγ, indicated with Th1 response which is confirmed by high level of IgG2a. These data demonstrate that the cocktailGRA7/ROP2 is a potential vaccine candidate against toxoplasmosis.

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APA: Copy

Vazini, Hossein, GHAFARIFAR, FATEMEH, SHARIFI, ZOHREH, & Dalimi, Abdolhosein. (2018). EVALUATION OF IMMUNE RESPONSES INDUCED BY GRA7 AND ROP2 GENES BY DNA VACCINE COCKTAILS AGAINST ACUTE TOXOPLASMOSIS IN BALB/C MICE. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 10(1), 2-8. SID. https://sid.ir/paper/313943/en

Vancouver: Copy

Vazini Hossein, GHAFARIFAR FATEMEH, SHARIFI ZOHREH, Dalimi Abdolhosein. EVALUATION OF IMMUNE RESPONSES INDUCED BY GRA7 AND ROP2 GENES BY DNA VACCINE COCKTAILS AGAINST ACUTE TOXOPLASMOSIS IN BALB/C MICE. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2018;10(1):2-8. Available from: https://sid.ir/paper/313943/en

IEEE: Copy

Hossein Vazini, FATEMEH GHAFARIFAR, ZOHREH SHARIFI, and Abdolhosein Dalimi, “EVALUATION OF IMMUNE RESPONSES INDUCED BY GRA7 AND ROP2 GENES BY DNA VACCINE COCKTAILS AGAINST ACUTE TOXOPLASMOSIS IN BALB/C MICE,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 10, no. 1, pp. 2–8, 2018, [Online]. Available: https://sid.ir/paper/313943/en

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