ALPHA ALUMINA NANOPARTICLE CONJUGATION TO CYSTEINE PEPTIDASE A AND B: AN EFFICIENT METHOD FOR AUTOPHAGY INDUCTION

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BEYZAY FATEMEH; ZAVARAN HOSSEINI AHMAD; SOUDI SARA

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Journal Paper

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Journal: Avicenna Journal of Medical Biotechnology Year:2017 | Volume:9 | Issue:2 Page(s): 71-81

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Title

ALPHA ALUMINA NANOPARTICLE CONJUGATION TO CYSTEINE PEPTIDASE A AND B: AN EFFICIENT METHOD FOR AUTOPHAGY INDUCTION

Author(s)

BEYZAY FATEMEH | ZAVARAN HOSSEINI AHMAD | SOUDI SARA | Issue Writer Certificate

Keywords

AUTOPHAGY

CYSTEINE PEPTIDASE

MACROPHAGE

Abstract

Background: AUTOPHAGY as a cellular pathway facilitates several immune responses against infection. It also eliminates invading pathogens through transferring content between the cytosol and the lysosomal vesicles and contributes to the cross-presentation of exogenous antigens to T lymphocytesvia MHC class I pathway. AUTOPHAGY induction is one of the main targets for new drugs and future vaccine formulations.Nanoparticles are one of the candidates for AUTOPHAGY induction. CYSTEINE PEPTIDASE A (CPA) and CYSTEINE PEPTIDASE B (CPB) are two members of papain family (Clan CA, family C1) enzyme that have been considered as a virulence factor of Leishmania (L.) major, making them suitable vaccine candidates. In this research, Leishmania major CYSTEINE PEPTIDASE A and B (CPA and CPB) conjugation to alpha alumina nanoparticle was the main focus and their entrance efficacy to MACROPHAGEs was assessed.Methods: For this purpose, CPA and CPB genes were cloned in expression vectors. Related proteins were extracted from transformed Escherichia coli (E. coli) and purified using Ni affinity column. Alpha alumina nanoparticles were conjugated to CPA/CPB proteins using Aldehyde/Hydrazine Reaction. AUTOPHAGY induction in MACROPHAGEs was assessed using acridine orange staining.Results: CPA/CPB protein loading to nanoparticles was confirmed by Fourier Transform Infrared Spectroscopy.α-alumina conjugated CPA/CPB antigen uptake by MACROPHAGEs at different concentrations was confirmed using fluorescence microscope and flow cytometry. Highly efficient CPA/CPB protein loading toα-alumina nanoparticles and rapid internalization to MACROPHAGEs introduced these nanocarriers as a delivery tool. Acridine orange staining demonstrated higher AUTOPHAGY induction in CPA/CPB protein conjugated withα-alumina nanoparticles.Conclusion: a-alumina nanoparticles may be a promising adjuvant in the development of therapeutic leishmania vaccines through antigen delivery to intracellular compartments, induction of AUTOPHAGY and cross presentation to CD8 lymphocytes.

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APA: Copy

BEYZAY, FATEMEH, ZAVARAN HOSSEINI, AHMAD, & SOUDI, SARA. (2017). ALPHA ALUMINA NANOPARTICLE CONJUGATION TO CYSTEINE PEPTIDASE A AND B: AN EFFICIENT METHOD FOR AUTOPHAGY INDUCTION. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 9(2), 71-81. SID. https://sid.ir/paper/313979/en

Vancouver: Copy

BEYZAY FATEMEH, ZAVARAN HOSSEINI AHMAD, SOUDI SARA. ALPHA ALUMINA NANOPARTICLE CONJUGATION TO CYSTEINE PEPTIDASE A AND B: AN EFFICIENT METHOD FOR AUTOPHAGY INDUCTION. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2017;9(2):71-81. Available from: https://sid.ir/paper/313979/en

IEEE: Copy

FATEMEH BEYZAY, AHMAD ZAVARAN HOSSEINI, and SARA SOUDI, “ALPHA ALUMINA NANOPARTICLE CONJUGATION TO CYSTEINE PEPTIDASE A AND B: AN EFFICIENT METHOD FOR AUTOPHAGY INDUCTION,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 9, no. 2, pp. 71–81, 2017, [Online]. Available: https://sid.ir/paper/313979/en

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