EFFICIENT MEDIA FOR HIGH LIPASE PRODUCTION: ONE VARIABLE AT A TIME APPROACH

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SOLEYMANI SAFOURA; ALIZADEH HOURI; MOHAMMADIAN HOSSEIN; RABBANI EMAD; MOAZEN FATEMEH; MIRMOHAMMAD SADEGHI HAMID; SAMSAM SHARIAT ZIAEDIN; ETEMADIFAR ZAHRA; RABBANI MOHAMMED

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Journal: Avicenna Journal of Medical Biotechnology Year:2017 | Volume:9 | Issue:2 Page(s): 82-86

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Title

EFFICIENT MEDIA FOR HIGH LIPASE PRODUCTION: ONE VARIABLE AT A TIME APPROACH

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Keywords

BASILLUS PUMILUS ZR-5

CULTURE MEDIA

LIPASE ENZYME

Abstract

Background: LIPASE ENZYMEs have applications in a wide range of industries. A crucial determining factor of industrial prices of these enzymes is the CULTURE MEDIA composition that is constantly under review by researchers. In this work, for maximum lipase production byBacillus sp. ZR-5, CULTURE MEDIA compositions were optimized using "one variable at a time" strategy.Methods: For this purpose, the culture medium parameters such as low and high cost carbon and nitrogen sources, substrates and incubation times were evaluated.Results: Maximum lipase activity was achieved after 24 hr of incubation with 1.5% of glucose syrup (1600±69.1u/mg), 1% of fish powder (1238±36.7 u/mg) and olive oil (1407±2.1u/mg) as low cost carbon and nitrogen sources and substrate, respectively.Conclusion: Our results show a significant increase in lipase activity with usage of low cost sources; this could help in reducing the media prices for industrial application of LIPASE ENZYME.

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APA: Copy

SOLEYMANI, SAFOURA, ALIZADEH, HOURI, MOHAMMADIAN, HOSSEIN, RABBANI, EMAD, MOAZEN, FATEMEH, MIRMOHAMMAD SADEGHI, HAMID, SAMSAM SHARIAT, ZIAEDIN, ETEMADIFAR, ZAHRA, & RABBANI, MOHAMMED. (2017). EFFICIENT MEDIA FOR HIGH LIPASE PRODUCTION: ONE VARIABLE AT A TIME APPROACH. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 9(2), 82-86. SID. https://sid.ir/paper/313985/en

Vancouver: Copy

SOLEYMANI SAFOURA, ALIZADEH HOURI, MOHAMMADIAN HOSSEIN, RABBANI EMAD, MOAZEN FATEMEH, MIRMOHAMMAD SADEGHI HAMID, SAMSAM SHARIAT ZIAEDIN, ETEMADIFAR ZAHRA, RABBANI MOHAMMED. EFFICIENT MEDIA FOR HIGH LIPASE PRODUCTION: ONE VARIABLE AT A TIME APPROACH. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2017;9(2):82-86. Available from: https://sid.ir/paper/313985/en

IEEE: Copy

SAFOURA SOLEYMANI, HOURI ALIZADEH, HOSSEIN MOHAMMADIAN, EMAD RABBANI, FATEMEH MOAZEN, HAMID MIRMOHAMMAD SADEGHI, ZIAEDIN SAMSAM SHARIAT, ZAHRA ETEMADIFAR, and MOHAMMED RABBANI, “EFFICIENT MEDIA FOR HIGH LIPASE PRODUCTION: ONE VARIABLE AT A TIME APPROACH,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 9, no. 2, pp. 82–86, 2017, [Online]. Available: https://sid.ir/paper/313985/en

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