Preconditioning with SDF-1 Improves Therapeutic Outcomes of Bone marrow-derived Mesenchymal Stromal Cells in a Mouse Model of STZ-induced Diabetes

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GHOLAMI FARASHAHI MOHAMMAD SADEGH; PASBAKHSH PARICHEHR; OMIDI AMENEH; NEKOONAM SAEID; Aryanpour Roya; RAGERDI KASHANI IRAJ

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Journal: Avicenna Journal of Medical Biotechnology Year:2019 | Volume:11 | Issue:1 Page(s): 35-42

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Title

Preconditioning with SDF-1 Improves Therapeutic Outcomes of Bone marrow-derived Mesenchymal Stromal Cells in a Mouse Model of STZ-induced Diabetes

Author(s)

Keywords

Bone marrow-derived mesenchymal stromal cells

Diabetes mellitus

Homing

SDF-1

Abstract

Background: Nowadays, transplantation of Bone marrow-derived mesenchymal stromal cells (BMSCs) is currently an important alternative therapy for patient’ s type 1 Diabetes mellitus. But a number of critical obstacles lie ahead of this new strategy including reducing stem cell Homing to the damaged tissue due to oxidative stress. The purpose of the present study was to investigate whether preconditioning of BMSCs with SDF-1 could enhance their Homing to the pancreas and promote regeneration of the pancreatic β cells after being intravenously injected. Methods: Mice BMSCs were isolated and expanded. Cell proliferation was assayed by MTT Assay. Preconditioning was performed with 10 ng/ml SDF-1α for 24 hr. Male NMRI mice were injected with high-dose STZ (150 mg/kg). The preconditioned or unpreconditioned BMSCs at a dose of 1×106 cells were infused via the tail vein. Blood and pancreatic tissue samples were taken from all mice for flow cytometry, biochemical and histological studies. Results: Proliferation and Homing of BMSCs to the pancreas were significantly increased in the BMSCs with SDF-1α preconditioning. Differentiation of transplanted BMSCs, were significantly increased in preconditioning group. Although BMSCs without SDF-1 preconditioning exhibited remarkable recovery of pancreatic islets structure but this recovery were significantly increased in the BMSCs with SDF-1α preconditioning. Conclusion: Our results showed the effectiveness of SDF-1α preconditioning in BMSCs transplantation of STZ induced diabetes mice which might be achieved through improvement of BMSCs Homing into the injured pancreas.

Cites

Protective Effect of Fumaria parviflora Extract on Oxidative Stress and Testis Tissue Damage in Diabetic Rats

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APA: Copy

GHOLAMI FARASHAHI, MOHAMMAD SADEGH, PASBAKHSH, PARICHEHR, OMIDI, AMENEH, NEKOONAM, SAEID, Aryanpour, Roya, & RAGERDI KASHANI, IRAJ. (2019). Preconditioning with SDF-1 Improves Therapeutic Outcomes of Bone marrow-derived Mesenchymal Stromal Cells in a Mouse Model of STZ-induced Diabetes. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 11(1), 35-42. SID. https://sid.ir/paper/314010/en

Vancouver: Copy

GHOLAMI FARASHAHI MOHAMMAD SADEGH, PASBAKHSH PARICHEHR, OMIDI AMENEH, NEKOONAM SAEID, Aryanpour Roya, RAGERDI KASHANI IRAJ. Preconditioning with SDF-1 Improves Therapeutic Outcomes of Bone marrow-derived Mesenchymal Stromal Cells in a Mouse Model of STZ-induced Diabetes. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2019;11(1):35-42. Available from: https://sid.ir/paper/314010/en

IEEE: Copy

MOHAMMAD SADEGH GHOLAMI FARASHAHI, PARICHEHR PASBAKHSH, AMENEH OMIDI, SAEID NEKOONAM, Roya Aryanpour, and IRAJ RAGERDI KASHANI, “Preconditioning with SDF-1 Improves Therapeutic Outcomes of Bone marrow-derived Mesenchymal Stromal Cells in a Mouse Model of STZ-induced Diabetes,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 11, no. 1, pp. 35–42, 2019, [Online]. Available: https://sid.ir/paper/314010/en

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