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Information Journal Paper

Title

Evaluation of Differential Gene Expression during Transdifferentiation of Bone Marrow Stromal Cells to Glial Phenotype in the Presence of Cerebrospinal Fluid

Pages

  28-34

Abstract

 Background: The present study assessed the alteration of gene expression during transdifferentiation of Cells/fa?page=1&sort=1&ftyp=all&fgrp=all&fyrs=all" target="_blank">Bone marrow stromal Cells (BMSCs) into Oligodendrocyte in the presence of Cerebrospinal fluid (CSF). Methods: BMSCs were collected from female Sprague-Dawley rats and were cultured in DMEM/F12 medium supplemented with Retinoic Acid (RA), basic Fibroblast Growth Factor (bFGF), and Epidermal Growth Factor (EGF). CSF was added daily to the culture media. The Oligoprogenitor and Oligodendrocyte generation was assessed by immunocytochemistry for Oligo 2, A2B5, CNP and MBP markers. Results: The mean percentages of immunopositive Cells for Olig2 and A2B5 were 52. 1± 1. 74 and 56. 34± 2. 55%, respectively. The number of immunopositive Cells for glial markers CNP and MBP were 48. 8± 3. 12 and 40. 5± 8. 92%, respectively. Alteration of gene expression of Oct4, Olig 2, PDGFR-α and PLP were examined by real time PCR during transdifferentiation of BMSC to Oligodendrocyte. Immunocytochemical results indicate that Oligoprogenitor Cells were immunopositive for Oligo2 and A2B5 markers. Also, Oligodendrocytes expressed the mature glial markers of CNP and MBP indicating successful differentiation. Conclusion: In conclusion, CSF promotes the transdifferentiation of BMSC into mature Oligodendrocyte via providing an appropriate niche for glial maturation.

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    Cite

    APA: Copy

    GHASEMI HAMIDABADI, HATEF, NAZM BOJNORDI, MARYAM, REZAEI, NOUROLLAH, & SOLEIMANI, SARA. (2019). Evaluation of Differential Gene Expression during Transdifferentiation of Bone Marrow Stromal Cells to Glial Phenotype in the Presence of Cerebrospinal Fluid. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 11(1), 28-34. SID. https://sid.ir/paper/314019/en

    Vancouver: Copy

    GHASEMI HAMIDABADI HATEF, NAZM BOJNORDI MARYAM, REZAEI NOUROLLAH, SOLEIMANI SARA. Evaluation of Differential Gene Expression during Transdifferentiation of Bone Marrow Stromal Cells to Glial Phenotype in the Presence of Cerebrospinal Fluid. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2019;11(1):28-34. Available from: https://sid.ir/paper/314019/en

    IEEE: Copy

    HATEF GHASEMI HAMIDABADI, MARYAM NAZM BOJNORDI, NOUROLLAH REZAEI, and SARA SOLEIMANI, “Evaluation of Differential Gene Expression during Transdifferentiation of Bone Marrow Stromal Cells to Glial Phenotype in the Presence of Cerebrospinal Fluid,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 11, no. 1, pp. 28–34, 2019, [Online]. Available: https://sid.ir/paper/314019/en

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