Evaluation of the Effect of Morphine and Imiquimodon Expression of TLR2 and TLR4 from Lesion RNA Extracted from BALB/c Mice Infected with Leishmania major; Short Communication

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EBRAHIMISADR PARISA; GHAFFARIFAR FATEMEH; HORTON JOHN; DALIMI ABDOLHOSSEIN; SHARIFI ZOHREH

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Journal: Avicenna Journal of Medical Biotechnology Year:2019 | Volume:11 | Issue:2 Page(s): 202-205

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Title

Evaluation of the Effect of Morphine and Imiquimodon Expression of TLR2 and TLR4 from Lesion RNA Extracted from BALB/c Mice Infected with Leishmania major; Short Communication

Author(s)

Keywords

Leishmania major

Lesions

Mice

Morphine

Toll-like receptors

Abstract

Background: Toll-like receptors (TLRs) are the cause of phagocytosis activation and destruction of the infection agents. In addition, new evidences support the idea that TLRs play a vital role in starting the acquired immunity reactions. Methods: In this study, it has been attempted to infect the BALB/c Mice with Leish-mania major (L. major) and treat them using Morphine and imiquimod; then the ex-pressions of TLR2, 4 from treated lesion were studied by using Real-Time PCR method. Treatment with Morphine 1 mg/kg, imiquimod 5% and nalmefene 1 mg/kg began four weeks after the challenge. After treatment period, half of the Mice of each group were killed and their Lesions were isolated for RNA extraction and making cDNA. For the rest of Mice, lesion size was measured weekly. Results: The results showed increase of expression of TLR2 gene among all treated groups relative to the control, and the difference was significant (p<0. 05). The expres-sion of TLR4 gene only was reduced in groups under treatment with Morphine and Morphine plus nalmefene relative to the control group and in the other groups in-creased. The highest expression of TLR2 was seen in the group treated by glucantime (p<0. 0001). Conclusion: However, in this study it was found that despite decreasing the size of le-sion in all treated groups, expression of TLR4 in the Morphine, nalmefene, Morphine plus nalmefene treated groups compared to the control group was decreased. There-fore, Morphine may have a different function mechanism in treatment of the Leish-maniasis with the L. major.

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References

EFFECT OF POLYVINYL ALCOHOL (PVA) CONTAINING ARTEMETHER IN TREATMENT OF CUTANEOUS LEISHMANIASIS CAUSED BY LEISHMANIA MAJOR IN BALB/C MICE

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APA: Copy

EBRAHIMISADR, PARISA, GHAFFARIFAR, FATEMEH, HORTON, JOHN, DALIMI, ABDOLHOSSEIN, & SHARIFI, ZOHREH. (2019). Evaluation of the Effect of Morphine and Imiquimodon Expression of TLR2 and TLR4 from Lesion RNA Extracted from BALB/c Mice Infected with Leishmania major; Short Communication. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 11(2), 202-205. SID. https://sid.ir/paper/314025/en

Vancouver: Copy

EBRAHIMISADR PARISA, GHAFFARIFAR FATEMEH, HORTON JOHN, DALIMI ABDOLHOSSEIN, SHARIFI ZOHREH. Evaluation of the Effect of Morphine and Imiquimodon Expression of TLR2 and TLR4 from Lesion RNA Extracted from BALB/c Mice Infected with Leishmania major; Short Communication. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2019;11(2):202-205. Available from: https://sid.ir/paper/314025/en

IEEE: Copy

PARISA EBRAHIMISADR, FATEMEH GHAFFARIFAR, JOHN HORTON, ABDOLHOSSEIN DALIMI, and ZOHREH SHARIFI, “Evaluation of the Effect of Morphine and Imiquimodon Expression of TLR2 and TLR4 from Lesion RNA Extracted from BALB/c Mice Infected with Leishmania major; Short Communication,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 11, no. 2, pp. 202–205, 2019, [Online]. Available: https://sid.ir/paper/314025/en

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