مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

288
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

421
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

Optimization of Fermentation Conditions for Reteplase Expression by Escherichia coli Using Response Surface Methodology

Pages

  162-168

Abstract

 Background: Expression of heterologous proteins at large scale is often a challenging job due to plasmid instability, accumulation of acetate and oxidative damage in bio-reactors. Therefore, it is necessary to optimize parameters influencing cell growth and expression of recombinant protein. Methods: In the present study, the optimal culture conditions for expression of retep-lase by Escherichia coli (E. coli) BL21 (DE3) in a bench-top bioreactor was determined. Response Surface Methodology (RSM) based on Box-Behnken design was used to evaluate the effect of three variables (i. e., temperature, shaking speed and pH) and their interactions with cellular growth and protein production. The obtained data were analyzed by Design Expert software. Results: Based on results of 15 experiments, a response surface quadratic model was developed which was used to explain the relation between production of Reteplase and three investigated variables. The high value of "R-Squared" (0. 9894) and F-value of 51. 99 confirmed the accuracy of this model. According to the developed model, the optimum Fermentation conditions for Reteplase expression were temperature of 32° C, shaking speed of 210 rpm, and pH of 8. 4. This predicted condition was applied for the production of Reteplase in the bioreactor leading to a protein yield of 188 mg/l. Conclusion: Our results indicate the significant role of culture conditions (e. g., pH, temperature and oxygen supply) in protein expression at large scale and confirm the need for optimization. The proposed strategy here can also be applied to experi-mental set-up of optimization for Fermentation of other proteins.

Cites

  • No record.

    • References

    Cite

    APA: Copy

    Zare, Hamze, MIR MOHAMMAD SADEGHI, HAMID, & AKBARI, VAJIHE. (2019). Optimization of Fermentation Conditions for Reteplase Expression by Escherichia coli Using Response Surface Methodology. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 11(2), 162-168. SID. https://sid.ir/paper/314030/en

    Vancouver: Copy

    Zare Hamze, MIR MOHAMMAD SADEGHI HAMID, AKBARI VAJIHE. Optimization of Fermentation Conditions for Reteplase Expression by Escherichia coli Using Response Surface Methodology. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2019;11(2):162-168. Available from: https://sid.ir/paper/314030/en

    IEEE: Copy

    Hamze Zare, HAMID MIR MOHAMMAD SADEGHI, and VAJIHE AKBARI, “Optimization of Fermentation Conditions for Reteplase Expression by Escherichia coli Using Response Surface Methodology,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 11, no. 2, pp. 162–168, 2019, [Online]. Available: https://sid.ir/paper/314030/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button