Cloning and Expression of B. mellitensis bp26 Gene in Lactococcus lactis as a Food Grade Vaccine

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Azizpour Maghvan Maryam; JAFARI PARVANEH; HOSEINI SEYED DAVOOD; BEHROZIKHAH ALI MOHAMMAD

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Journal: Avicenna Journal of Medical Biotechnology Year:2019 | Volume:11 | Issue:3 Page(s): 264-267

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Title

Cloning and Expression of B. mellitensis bp26 Gene in Lactococcus lactis as a Food Grade Vaccine

Author(s)

Azizpour Maghvan Maryam | JAFARI PARVANEH | HOSEINI SEYED DAVOOD | BEHROZIKHAH ALI MOHAMMAD | Issue Writer Certificate

Keywords

Brucellosis

Exotoxins

Lactococcus lactis

Vaccines

Abstract

Background: Brucellosis is still an important health problem in under developing countries and researches for finding efficient vaccine are going on. Brucella melitensis (B. mellitensis) bp26 gene is a good candidate for Brucellosis vaccine and investiga-tions showed that Lactococcus lactis (L. lactis) with several positive characteristic are attractive for protein expression as a live delivery vectors. These fast growing bacteria need no aeration, are easy to handle, have no exotoxin, endotoxin and protease, so the cost of culturing is inexpensive. Methods: B. mellitensis bp26 gene was cloned in food grade pNZ 8149 vector and ex-pressed in L. lactis NZ 3900. Results: Results showed that we can produce a food-grade recombinant L. lactis pro-ducing the B. melitensis BP26 protein. Conclusion: In this study, for Future evaluation about ability of L. lactis as a live deliv-ery vector, a food-grade recombinant L. lactis producing the B. melitensis BP26 pro-tein was produced.

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APA: Copy

Azizpour Maghvan, Maryam, JAFARI, PARVANEH, HOSEINI, SEYED DAVOOD, & BEHROZIKHAH, ALI MOHAMMAD. (2019). Cloning and Expression of B. mellitensis bp26 Gene in Lactococcus lactis as a Food Grade Vaccine. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 11(3), 264-267. SID. https://sid.ir/paper/314036/en

Vancouver: Copy

Azizpour Maghvan Maryam, JAFARI PARVANEH, HOSEINI SEYED DAVOOD, BEHROZIKHAH ALI MOHAMMAD. Cloning and Expression of B. mellitensis bp26 Gene in Lactococcus lactis as a Food Grade Vaccine. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2019;11(3):264-267. Available from: https://sid.ir/paper/314036/en

IEEE: Copy

Maryam Azizpour Maghvan, PARVANEH JAFARI, SEYED DAVOOD HOSEINI, and ALI MOHAMMAD BEHROZIKHAH, “Cloning and Expression of B. mellitensis bp26 Gene in Lactococcus lactis as a Food Grade Vaccine,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 11, no. 3, pp. 264–267, 2019, [Online]. Available: https://sid.ir/paper/314036/en

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