مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

210
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

153
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

Design of Anti-Angiogenic Peptidomimetics and Evaluation their Biological Activity by In Vitro Assays

Pages

  91-98

Abstract

 Background: One of the important therapeutic approaches in cancer field is development of compounds which can block the initial tumor growth and the progression of tumor metastasis with no side effects. Thus, the recent study was carried out to design anti-VEGFR2-Peptidomimetics as the most significant factor of angiogenesis process-and evaluate their biological activity by in vitro assays. Methods: We designed anti-VEGFR2 Peptidomimetics with anti-angiogenic activity, including compound P (lactam derivative) and compound T (indole derivative) by using in silico methods. Then, the inhibitory activity on angiogenesis was evaluated by using angiogenesis specific assays such as Human Umbilical Vein Endothelial Cell (HUVEC) proliferation, tube formation in Matrigel, MTT and Real-Time PCR. IC50 values of the compounds were also determined by cytotoxicity plot in MTT assay. Results: Compounds P and T inhibited HUVEC cell proliferation and viability in a dose-dependent manner. The IC50 for compound T and compound P in HUVEC cell line were 113 and 115 μ g/ml, respectively. Tube formation assay revealed that both compounds can inhibit angiogenesis effectively. The results of Real-Time PCR also showed these compounds are able to inhibit the expression of CD31 gene in HUVEC cell line. Conclusion: Our study suggested that compounds P and T may act as therapeutic molecules, or lead compounds for development of Angiogenesis Inhibitors in VEGFrelated diseases.

Cites

  • No record.

    • References

    Cite

    APA: Copy

    Ghadam, Mona, SARDARI, SOROUSH, SHOKRGOZAR, MOHAMMAD ALI, & Mahdavi, Mahdiyeh Sadat. (2020). Design of Anti-Angiogenic Peptidomimetics and Evaluation their Biological Activity by In Vitro Assays. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 12(2), 91-98. SID. https://sid.ir/paper/314076/en

    Vancouver: Copy

    Ghadam Mona, SARDARI SOROUSH, SHOKRGOZAR MOHAMMAD ALI, Mahdavi Mahdiyeh Sadat. Design of Anti-Angiogenic Peptidomimetics and Evaluation their Biological Activity by In Vitro Assays. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2020;12(2):91-98. Available from: https://sid.ir/paper/314076/en

    IEEE: Copy

    Mona Ghadam, SOROUSH SARDARI, MOHAMMAD ALI SHOKRGOZAR, and Mahdiyeh Sadat Mahdavi, “Design of Anti-Angiogenic Peptidomimetics and Evaluation their Biological Activity by In Vitro Assays,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 12, no. 2, pp. 91–98, 2020, [Online]. Available: https://sid.ir/paper/314076/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button